Osteogenic properties of human myogenic progenitor cells
Osteogenic properties of human myogenic progenitor cells
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DOI：
10.1016/j.mod.2007.11.004
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发表时间：
2008-03-01
影响因子：
2.6
通讯作者：
Inagawa-Ogashiwa, Masayo
中科院分区：
文献类型：
--
作者：
Hashimoto, Naohiro;Kiyono, Tohru;Inagawa-Ogashiwa, Masayo
在这里，我们确定了人肌源性祖细胞共表达Pax7，肌肉卫星细胞的标志物和骨特异性碱性磷酸酶，成骨细胞的标志物，在再生肌肉。为了确定人肌源性祖细胞是否能够作为骨祖细胞，我们培养了来自非营养不良妇女健康肌肉的原代和永生化祖细胞。未分化的成肌祖细胞自发表达两种成骨细胞特异性蛋白，骨特异性碱性磷酸酶和Runx 2，并且能够在不暴露于外源性诱导剂如骨形态发生蛋白的情况下进行终末成骨分化。他们还表达了肌肉谱系特异性蛋白Pax7和MyoD，并失去了与终末肌肉分化相关的成骨特性。因此，成肌细胞和成骨细胞的特性在人肌源性细胞谱系中同时表达，然后致力于肌肉分化。此外，C3转移酶，一种特异性的Rho GT3抑制剂，阻断了人肌源性祖细胞的成肌分化，但不阻断其成骨分化。这些数据表明，人肌源性祖细胞保留了作为自发形成异位骨的骨祖细胞的能力，并且Rho信号传导参与了人肌源性细胞谱系中肌生成和骨生成之间的关键转换。(C)2007爱思唯尔爱尔兰有限公司保留所有权利。
Here, we identified human myogenic progenitor cells coexpressing Pax7, a marker of muscle satellite cells and bone-specific alkaline phosphatase, a marker of osteoblasts, in regenerating muscle. To determine whether human myogenic progenitor cells are able to act as osteoprogenitor cells, we cultured both primary and immortalized progenitor cells derived from the healthy muscle of a nondystrophic woman. The undifferentiated myogenic progenitors spontaneously expressed two osteoblast-specific proteins, bone-specific alkaline phosphatase and Runx2, and were able to undergo terminal osteogenic differentiation without exposure to an exogenous inductive agent such as bone morphogenetic proteins. They also expressed the muscle lineage-specific proteins Pax7 and MyoD, and lost their osteogenic characteristics in association with terminal muscle differentiation. Both myoblastic and osteoblastic properties are thus simultaneously expressed in the human myogenic cell lineage prior to commitment to muscle differentiation. In addition, C3 transferase, a specific inhibitor of Rho GTPase, blocked myogenic but not osteogenic differentiation of human myogenic progenitor cells. These data suggest that human myogenic progenitor cells retain the capacity to act as osteoprogenitor cells that form ectopic bone spontaneously, and that Rho signaling is involved in a critical switch between myogenesis and osteogenesis in the human myogenic cell lineage. (C) 2007 Elsevier Ireland Ltd. All rights reserved.