A spectral imaging array biosensor and its application in detection of leukemia cell
A spectral imaging array biosensor and its application in detection of leukemia cell
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DOI：
10.1016/j.snb.2011.02.035
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发表时间：
2011-08-01
影响因子：
8.4
通讯作者：
Yang, Yucong
中科院分区：
文献类型：
--
作者：
Fang, Xiangyi;Liu, Chunlong;Yang, Yucong
将表面等离子体共振（SPR）技术与光谱成像技术相结合，研制了一种新型的阵列免疫传感器。该系统由单色器作为波长扫描光源、偏振器、包括阵列传感器芯片的Kretschmann-Raaether衰减全反射（ATR）结构和CCD相机组成。记录ATR透射光随波长变化的图像。通过对来自图像序列的每个金斑点的区域中的像素的灰度进行平均，可以并行地提取阵列上的所有感测斑点的完整光谱共振曲线。通过分析抗CD 33单克隆抗体与其靶白血病细胞的相互作用，使用11例人骨髓标本，评价了所开发系统的性能。同时采用流式细胞术（FCM）进行分析比较。免疫传感器芯片的初步检测结果与流式细胞仪检测结果一致，表明该平行检测方法可用于急性白血病的免疫分型。该传感器阵列系统具有高通量、高灵敏度、高特异性、免标记、操作方便等优点。如果采用合适的技术来操纵传感器阵列芯片上的微生物液体，可以增加阵列的点数。(C)2011 Elsevier B. V.保留所有权利。
A new type of array immunosensor was developed by combining surface plasmon resonance (SPR) and spectral imaging techniques. The system consisted of a monochromator as the wavelength scanning light source, a polarizer, Kretschmann-Raaether attenuated total reflection (ATR) configuration including array sensor chip, and a CCD camera. The images of transmitting light from ATR were recorded versus the wavelength. By averaging gray scales of the pixels in the area of every gold spot from the image series, the complete spectral resonance curve of all sensing spots on the array can be extracted in parallel. The performance of the developed system was evaluated by analyzing interactions of the anti-CD33 monoclonal antibody to its target leukemic cells using 11 cases of human bone marrow specimens. The specimens were also analyzed with flow cytometry method (FCM) for comparison. The initial results measured by the immunosensor array were corresponded with that of FCM, indicating that the developed parallel method might be clinically suitable for immunophenotyping of acute leukemias. The new sensor array system showed the merits of high-throughput, high sensitivity, high specificity, label free and operation convenient. Spots numbers of the array could be increased if suitable technology were adopted for manipulating the micro bio-liquids on the sensor array chip. (C) 2011 Elsevier B.V. All rights reserved.