IMMUNOCYTOCHEMICAL STAINING OF LYMPHOCYTE-T AND LYMPHOCYTE-B IN SEROUS EFFUSIONS
IMMUNOCYTOCHEMICAL STAINING OF LYMPHOCYTE-T AND LYMPHOCYTE-B IN SEROUS EFFUSIONS
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DOI：
10.1136/jcp.38.6.608
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发表时间：
1985-01-01
影响因子：
3.4
通讯作者：
MASON, DY
中科院分区：
文献类型：
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作者：
GHOSH, AK;SPRIGGS, AI;MASON, DY
采用碱性磷酸酶-抗碱性磷酸酶技术，用一组单克隆抗体，包括抗B细胞抗体、抗T细胞抗体和抗HLA-DR，对含有大量淋巴细胞的浆液性渗出液的细胞涂片进行染色。例如癌症和心力衰竭的病例。反应性渗出液中的大多数淋巴细胞是T细胞，缺乏HLA-DR，并显示出明显过量的辅助/诱导细胞（平均辅助/抑制比为3.5）。9例B细胞瘤样淋巴细胞均为B细胞抗原和HLA-DR阳性，4例B细胞瘤样淋巴细胞以反应性T细胞为主。两例T细胞淋巴样白血病的特点也免疫细胞化学染色，都被归类为T辅助细胞肿瘤。对常规制备的风干细胞涂片进行标记，染色前将其在未固定状态下长期储存。因此，该技术可用于许多临床细胞学实验室诊断含有大量淋巴细胞的积液。
Cell smears from serous effusions containing large numbers of lymphoid cells were stained by the alkaline phosphatase-anti-alkaline phosphatase technique with a panel of monoclonal antibodies, including anti-B and anti-T cell antibodies and anti-HLA-DR. Samples from 17 patients with lymphoproliferative disorders, such as chronic lymphocytic leukemia and non-Hodgkin''s lymphoma, and from 19 patients who had no evidence of lymphoid neoplasia, for example, cases of carcinoma and cardiac failure, were investigated. The majority of lymphoid cells in reactive effusions were T cells, which lacked HLA-DR and showed a marked excess of helper/inducer cells (mean helper to suppressor ratio of 3.5). Lymphoid cells in samples from 9 cases of B cell neoplasia were positive for B cell antigen and HLA-DR. In a further 4 B cell neoplasms most lymphoid cells were reactive T cells. Two cases of T cell lymphoid leukemia were also characterized by immunocytochemical staining, both being classified as T helper cell neoplasms. Labeling was performed on routinely prepared, air dried cell smears, which were stored in the unfixed state for long periods before staining. The technique may therefore be of use in many clinical cytology laboratories for the diagnosis of effusions containing numerous lymphoid cells.