A SPECIFIC SUB-SET OF HOST-CELL MESSENGER-RNAS PRIME INFLUENZA-VIRUS MESSENGER-RNA SYNTHESIS
A SPECIFIC SUB-SET OF HOST-CELL MESSENGER-RNAS PRIME INFLUENZA-VIRUS MESSENGER-RNA SYNTHESIS
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DOI：
10.1016/0168-1702(84)90003-0
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发表时间：
1984-01-01
期刊：
影响因子：
5
通讯作者：
LAMB, RA
中科院分区：
文献类型：
--
作者：
SHAW, MW;LAMB, RA
比较从29个c[互补]DNA克隆序列获得的甲型和B流感病毒mRNA 5“末端的宿主细胞衍生RNA引物序列。这是对来自4种甲型和B型流感病毒株的5种不同基因组区段的克隆进行的。结果表明，在假定的核酸内切酶切割位点之前含有3“-末端Py-G-C-A序列的宿主RNA引物优选用作流感病毒mRNA合成中的引物。对体内合成的mRNA的5“-末端异质序列的引物延伸分析证实了对G-C-A-终止的引物片段的偏好，在A型和B型流感病毒基因组区段的转录物之间注意到一些差异。
The host-cell derived RNA primer sequences at the 5'' termini of mRNA of influenza A and B viruses, obtained from sequences of 29 c[complementary]DNA clones, were compared. This was done for clones of 5 different genome segments from 4 strains of influenza A and B virus. The results indicate that host RNA primers containing a 3''-terminal Py-G-C-A sequence before the presumed endonuclease cleavage site are preferred for use as primers in influenza virus mRNA synthesis. Primer-extension analyses of the 5''-terminal heterogeneous sequences of in vivo synthesized mRNA confirm the preference for G-C-A-terminated primer fragments, with some differences noted between the transcripts of types A and B influenza virus genome segments.