LC-Q-TOF-MS/MS detection of food flavonoids: principle, methodology, and applications
LC-Q-TOF-MS/MS detection of food flavonoids: principle, methodology, and applications
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食品类黄酮的 LC-Q-TOF-MS/MS 检测：原理、方法和应用
DOI：
10.1080/10408398.2021.1993128
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发表时间：
2021
影响因子：
10.2
通讯作者：
Jinkai Zheng
中科院分区：
文献类型：
--
作者：
Minke Yang;Juan Li;Chengying Zhao;Hang Xiao;Xiang Fang;Jinkai Zheng
类黄酮因其多种健康促进作用而吸引了越来越多的研究兴趣。然而，食品中存在许多结构相似的黄酮类化合物，但浓度往往较低，这增加了其分离和鉴定的难度。液相色谱-四极杆飞行时间串联质谱 (LC-Q-TOF-MS/MS) 已成为最广泛使用的类黄酮检测技术之一。 LC-Q-TOF-MS/MS可实现LC的高效分离；它还通过 Q-TOF-MS/MS 提供有关黄酮类化合物的结构信息。本综述全面总结了专门针对食品类黄酮的 LC-Q-TOF-MS/MS 的科学原理和详细方法（例如定性测定、定量测定和数据处理）。还讨论了LC-Q-TOF-MS/MS在农产品中黄酮类化合物类型和含量、食品加工过程中其结构和含量变化以及食用后体内代谢方面的最新应用。此外，它还提出了必要的技术改进和潜在的应用。该综述将促进人们对LC-Q-TOF-MS/MS黄酮类化合物检测理论和技术的科学认识，并促进其在食品和健康行业的应用。
Flavonoids have been attracting increasing research interest because of their multiple health promoting effects. However, many flavonoids with similar structures are present in foods, often at low concentrations, which increases the difficulty of their separation and identification. Liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (LC-Q-TOF-MS/MS) has become one of the most widely used techniques for flavonoid detection. LC-Q-TOF-MS/MS can achieve highly efficient separation by LC; it also provides structural information regarding flavonoids by Q-TOF-MS/MS. This review presents a comprehensive summary of the scientific principles and detailed methodologies (e.g., qualitative determination, quantitative determination, and data processing) of LC-Q-TOF-MS/MS specifically for food flavonoids. It also discusses the recent applications of LC-Q-TOF-MS/MS in determination of flavonoid types and contents in agricultural products, changes in their structures and contents during food processing, and metabolism in vivo after consumption. Moreover, it proposes necessary technological improvements and potential applications. This review would facilitate the scientific understanding of theory and technique of LC-Q-TOF-MS/MS for flavonoid detection, and promote its applications in food and health industry.