Multilineage differentiation of human mesenchymal stem cells in a three-dimensional nanofibrous scaffold
Multilineage differentiation of human mesenchymal stem cells in a three-dimensional nanofibrous scaffold
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DOI：
10.1016/j.biomaterials.2005.01.002
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发表时间：
2005-09-01
期刊：
影响因子：
14
通讯作者：
Tuan, RS
中科院分区：
文献类型：
--
作者：
Li, WJ;Tuli, R;Tuan, RS
肌肉骨骼组织的功能工程通常涉及在生物材料支架中使用种植有特定生长因子的分化或祖细胞。理想情况下，支架应该是天然细胞外基质的功能和结构仿生材料，并支持多种组织形态发生。我们之前已经证明，电纺的三维纳米纤维支架在形态上类似于胶原纤维，能够促进种子细胞的良好生物反应，这表明它们在组织工程中的潜在应用。在这项研究中，我们测试了一种由聚己内酯(PCL)构建的三维纳米纤维支架在体外支持和维持骨髓来源的人骨髓间充质干细胞(HMSCs)多向分化的能力。将hMSCs种植到预制的纳米纤维支架上，通过在特定的分化介质中培养，诱导其沿成脂、成软骨或成骨的方向分化。组织学和扫描电子显微镜观察、基因表达分析和谱系特异性标记分子的免疫组织化学检测证实形成了包含分化为特定细胞类型的细胞的三维结构。这些结果表明，基于PCL的纳米纤维支架是基于细胞的多相组织工程的一种有前途的候选支架。爱思唯尔有限公司出版。
Functional engineering of musculoskeletal tissues generally involves the use of differentiated or progenitor cells seeded with specific growth factors in biomaterial scaffolds. Ideally, the scaffold should be a functional and structural biomimetic of the native extracellular matrix and support multiple tissue morphogenesis. We have previously shown that electrospun, three-dimensional nanofibrous scaffolds that morphologically resemble collagen fibrils are capable of promoting favorable biological responses from seeded cells, indicative of their potential application for tissue engineering. In this study, we tested a three-dimensional nanofibrous scaffold fabricated from poly(e.-caprolactone) (PCL) for its ability to support and maintain multilineage differentiation of bone marrow-derived human mesenchymal stem cells (hMSCs) in vitro. hMSCs were seeded onto pre-fabricated nanofibrous scaffolds, and were induced to differentiate along adipogenic, chondrogenic, or osteogenic lineages by culturing in specific differentiation media. Histological and scanning electron microscopy observations, gene expression analysis, and immunohistochemical detection of lineage-specific marker molecules confirmed the formation of three-dimensional constructs containing cells differentiated into the specified cell types. These results suggest that the PCL-based nanofibrous scaffold is a promising candidate scaffold for cell-based, multiphasic tissue engineering. Published by Elsevier Ltd.