The rational design of nitric oxide selectivity in single-walled carbon nanotube near-infrared fluorescence sensors for biological detection
The rational design of nitric oxide selectivity in single-walled carbon nanotube near-infrared fluorescence sensors for biological detection
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DOI：
10.1038/nchem.332
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发表时间：
2009-09-01
期刊：
影响因子：
21.8
通讯作者：
Strano, Michael S.
中科院分区：
文献类型：
--
作者：
Kim, Jong-Ho;Heller, Daniel A.;Strano, Michael S.
合成纳米管或纳米线传感器的一个主要挑战是通过共价键以外的方法提供选择性的分析物结合，这会损害电子和光学性质。我们合成了一种用于单壁碳纳米管(SWNTs)的3，4-二氨基苯基功能化葡聚糖(DAP-Dex)包覆物，它可以快速和选择性地检测生物信号信使一氧化氮(NO)。SWNTDAP-Dex的近红外(NIR)荧光立即被NO直接漂白，而不被其他活性氮和氧物种漂白。这种漂白是可逆的，并被证明是由于电子从SWNT的价带顶部转移到NO的最低未占据分子轨道所致。由此产生的光学传感器能够实时和空间分辨地检测由刺激巨噬细胞中的一氧化氮合酶产生的一氧化氮。我们还展示了光学传感器在小鼠模型中体内检测NO的潜力。
A major challenge in the synthesis of nanotube or nanowire sensors is to impart selective analyte binding through means other than covalent linkages, which compromise electronic and optical properties. We synthesized a 3,4-diaminophenyl-functionalized dextran (DAP-dex) wrapping for single-walled carbon nanotubes (SWNTs) that imparts rapid and selective fluorescence detection of nitric oxide ( NO), a messenger for biological signalling. The near-infrared (nIR) fluorescence of SWNTDAP-dex is immediately and directly bleached by NO, but not by other reactive nitrogen and oxygen species. This bleaching is reversible and shown to be caused by electron transfer from the top of the valence band of the SWNT to the lowest unoccupied molecular orbital of NO. The resulting optical sensor is capable of real-time and spatially resolved detection of NO produced by stimulating NO synthase in macrophage cells. We also demonstrate the potential of the optical sensor for in vivo detection of NO in a mouse model.