Maturation-promoting factor induces nuclear envelope breakdown in cycloheximide-arrested embryos of Xenopus laevis.
Maturation-promoting factor induces nuclear envelope breakdown in cycloheximide-arrested embryos of Xenopus laevis.
复制标题
DOI：
10.1083/jcb.97.1.81
复制
发表时间：
1983-07
影响因子：
7.8
通讯作者：
Kirschner, M
中科院分区：
文献类型：
--
作者：
Miake-Lye, R;Newport, J;Kirschner, M
本文研究了成熟促进因子(MPF)对非洲爪哇早期卵裂胚胎细胞核的影响。当蛋白质合成在这一阶段被环己亚胺抑制时，胚胎细胞周期在完成另一轮DNA合成后停滞在人工产生的类似G2期的状态。如果首先用细胞松弛素B抑制胞质分裂，大约100个核可以被阻止在一个普通的细胞质中。在将MPF注射到这样的胚胎中后5分钟内，组织学或用抗层粘连蛋白抗血清对核层进行免疫荧光染色，发现每个核周围的核膜分散。核膜的破裂发生在与卵母细胞成熟所需的MPF相当或略低于的MPF水平。然而，MPF活性的放大并不像在卵母细胞中那样发生在受阻的卵子中。这些结果表明，MPF可以促进间期核进入有丝分裂的第一阶段，并表明核层对MPF的反应迅速。
We have studied the effect of maturation-promoting factor (MPF) on embryonic nuclei during the early cleavage stage of Xenopus laevis development. When protein synthesis is inhibited by cycloheximide during this stage, the embryonic cell cycle arrests in an artificially produced G2 phase-like state, after completion of one additional round of DNA synthesis. Approximately 100 nuclei can be arrested in a common cytoplasm if cytokinesis is first inhibited by cytochalasin B. Within 5 min after injection of MPF into such embryos, the nuclear envelope surrounding each nucleus disperses, as determined histologically or by immunofluorescent staining of the nuclear lamina with antilamin antiserum. The breakdown of the nuclear envelope occurs at levels of MPF comparable to or slightly lower than those required for oocyte maturation. Amplification of MPF activity, however, does not occur in the arrested egg as it does in the oocyte. These results suggest that MPF can act to advance interphase nuclei into the first events of mitosis and show that the nuclear lamina responds rapidly to MPF.