A GntR family transcriptional regulator (PigT) controls gluconate-mediated repression and defines a new, independent pathway for regulation of the tripyrrole antibiotic, prodigiosin, in Serratia
A GntR family transcriptional regulator (PigT) controls gluconate-mediated repression and defines a new, independent pathway for regulation of the tripyrrole antibiotic, prodigiosin, in Serratia
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DOI：
10.1099/mic.0.28251-0
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发表时间：
2005-12-01
期刊：
影响因子：
2.8
通讯作者：
Salmond, GPC
中科院分区：
文献类型：
--
作者：
Fineran, PC;Everson, L;Salmond, GPC
Serratia sp. ATCC 39006（39006）的红色三吡罗抗生素prodigiosin （Pig）的生物合成受到一个复杂的调控网络的控制，该网络涉及一个n -酰基高丝氨酸内酯（N-AHL）群体感应系统，至少两个独立的双组分信号转导系统和许多其他调节剂。在这项研究中，一种新的转录激活因子PigT和一种生理线索（葡萄糖酸盐）参与了控制猪生物合成的独立途径，并进行了表征。PigT是GntR的同系物，在没有葡萄糖酸盐的情况下激活pigA-O生物合成操纵子的转录。然而，在39006的生长培养基中添加葡萄糖酸盐，通过猪依赖机制抑制pigA-0的转录，导致猪产量下降。最后，猪t转录物的表达在指数期达到最大值，在猪开始生产之前。这项工作扩大了我们对影响39006猪次生代谢物生物合成的生理和遗传因素的理解。
Biosynthesis of the red, tripyrrole antibiotic prodigiosin (Pig) by Serratia sp. ATCC 39006 (39006) is controlled by a complex regulatory network involving an N-acyl homoserine lactone (N-AHL) quorum-sensing system, at least two separate two-component signal transduction systems and a multitude of other regulators. In this study, a new transcriptional activator, PigT, and a physiological cue (gluconate), which are involved in an independent pathway controlling Pig biosynthesis, have been characterized. PigT, a GntR homologue, activates transcription of the pigA-O biosynthetic operon in the absence of gluconate. However, addition of gluconate to the growth medium of 39006 repressed transcription of pigA-0, via a PigT-dependent mechanism, resulting in a decrease in Pig production. Finally, expression of the pigT transcript was shown to be maximal in exponential phase, preceding the onset of Pig production. This work expands our understanding of both the physiological and genetic factors that impinge on the biosynthesis of the secondary metabolite Pig in 39006.