Biodegradation of waste grease by Penicillium chrysogenum for production of fatty acid
Biodegradation of waste grease by Penicillium chrysogenum for production of fatty acid
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DOI：
10.1016/j.biortech.2016.12.006
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发表时间：
2017-02-01
影响因子：
11.4
通讯作者：
Khare, Sunil Kumar
中科院分区：
文献类型：
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作者：
Kumari, Arti;Ahmad, Razi;Khare, Sunil Kumar
目前工作的目的是有效修复产黄青霉造成的油脂废物。为了有效降解，使用各种脂肪酶对油脂废物进行预处理，其中脂肪酶效果最好。预处理的油脂被 P. chrysogenum 用作底物，从而产生脂肪酸。通过响应面法 (RSM) 使用四个变量优化流程： FeCl2 (mM)、孢子浓度 (孢子/ml)、时间段 (天) 和油脂量 (g)。优化的条件即； FeCl2 1.25 mM、培养量 5 x 10(11) 孢子/ml 和时间段 16 天，在固态发酵下，将 10.0 g 预处理油脂与 5.0 g 麦麸混合在 10.0 ml czapek-dox 培养基中，产生 6.6 mg/g 脂肪酸。用己烷提取发酵培养基并进行GCMS分析，结果显示存在较高量的棕榈酸。采用结晶法纯化，托盘发酵从1.0kg油脂废弃物中回收棕榈酸2.8g。 (C) 2016 Elsevier Ltd. 保留所有权利。
The aim of present work was to effectively remediate grease waste by Penicillium chrysogenum. For efficient degradation, grease waste was pre-treated using various lipases, among them lipolase was the best. The pretreated grease was used as a substrate by P. chrysogenum resulting into the production of fatty acids. Process was optimized by response surface methodology (RSM) using four variables viz; FeCl2 (mM), spore concentration (spores/ml), time period (days) and amount of grease (g). The optimized conditions viz; FeCl2 1.25 mM, culture amount 5 x 10(11) spores/ml and time period 16 days led to the production of 6.6 mg/g fatty acid from 10.0 g of pre-treated grease mixed with 5.0 g wheat bran in 10.0 ml czapek-dox medium under solid state fermentation. The fermented media was extracted with hexane and subjected to GCMS analysis, which showed the presence of higher amount of palmitic acid. It was purified by crystallization method and 2.8 g of palmitic acid was recovered from 1.0 kg grease waste in tray fermentation. (C) 2016 Elsevier Ltd. All rights reserved.