Utility of Green Fluorescent Nucleic Acid Dyes and Aluminum Oxide Membrane Filters for Rapid Epifluorescence Enumeration of Soil and Sediment Bacteria
Utility of Green Fluorescent Nucleic Acid Dyes and Aluminum Oxide Membrane Filters for Rapid Epifluorescence Enumeration of Soil and Sediment Bacteria
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绿色荧光核酸染料和氧化铝膜过滤器在土壤和沉积物细菌快速落射荧光计数中的应用
DOI：
10.1128/aem.64.12.5000-5003.1998
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发表时间：
1998
影响因子：
4.4
通讯作者：
M. Höfle
中科院分区：
文献类型：
--
作者：
M. Weinbauer;Christian Beckmann;M. Höfle
用4′，6-二脒基-2-苯基吲哚（DAPI）和聚碳酸酯滤膜对45%的土壤和沉积物样品进行细菌荧光计数时，由于背景荧光和非特异性染色的存在，使细菌荧光计数受到阻碍。通过使用绿色荧光高亲和力核酸染料和氧化铝膜过滤器，可以避免测定总细胞计数的这些问题。由于细胞染色明亮，我们推荐SYBR绿色II作为染料。
ABSTRACT High background fluorescence and unspecific staining hampered the epifluorescence enumeration of bacteria in 45% of the tested soil and sediment samples with 4′,6-diamidino-2-phenylindole (DAPI) and polycarbonate membrane filters. These problems of the determination of total cell counts can be circumvented by using green fluorescent high-affinity nucleic acid dyes and aluminum oxide membrane filters. Due to the bright staining of cells, we recommend SYBR Green II as dye.