Measurement of zinc stable isotope ratios in biogeochemical matrices by double-spike MC-ICPMS and determination of the isotope ratio pool available for plants from soil.
Measurement of zinc stable isotope ratios in biogeochemical matrices by double-spike MC-ICPMS and determination of the isotope ratio pool available for plants from soil.
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DOI：
10.1007/s00216-010-4231-5
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发表时间：
2010-12
影响因子：
4.3
通讯作者：
Weiss, Dominik J.
中科院分区：
文献类型：
--
作者：
Arnold, Tim;Schoenbaechler, Maria;Rehkaemper, Mark;Dong, Schuofei;Zhao, Fang-Jie;Kirk, Guy J. D.;Coles, Barry J.;Weiss, Dominik J.
关键词：
分析自然发生的同位素变化是一个很有前途的工具，调查锌运输和循环的地质和生物环境。在这里，我们提出了最近安装的双穗（DS）技术在魔法实验室在帝国理工学院伦敦。该程序改进了以前公布的DS方法的测量和精度方面的方便。分析方法包括在消化前向样品中加入64 Zn-67 Zn双加标物，通过离子交换色谱法从样品基质中分离Zn，并通过多收集器电感耦合等离子体质谱法进行同位素分析。该方法的准确性和重现性进行了验证，通过分析几个内部和国际元素参考材料。对纯Zn标准溶液进行多次分析，δ 66 Zn的重复性约为±0.05‰（2SD），对地质和生物样品的分析精度也相当。通过DS和标准样品交叉进样分析的高度分级分离的Zn标准品产生略微不同的结果，这可能源于标准品生产期间的重复分级分离事件。然而，两种分级较低的内部锌标准溶液Imperial Zn（0.10 ± 0.08‰：2 SD）和伦敦Zn（0.08 ± 0.04‰）的δ 66 Zn值（均相对于JMC里昂Zn报告）在不同质谱技术和仪器报告的数据的不确定度范围内。测定了混合矿BCR 027和黑麦草BCR 281两种标准物质的δ 66 Zn值分别为0.25 ± 0.06‰（2SD）和0.40 ± 0.09‰。两者合计，这些标准的测量确定，双穗方法是适合于准确和精确的锌同位素分析范围广泛的天然样品。因此，新安装的技术适用于土壤样品和土壤渗滤液，以调查植物有效锌的同位素签名。我们发现，同位素组成比残留重，表明存在松散结合的锌沉积的大气污染，这是很容易提供给植物。通过双加标MC-ICPMS测量的散装土壤和相关酸沥滤（估计的植物有效库）的锌同位素比库。δxZnLyon-JMC=（Rsample/RJMC-Lyon-1）× 103，其中Rsample和RJMC-Lyon分别表示样品和标准品（JMC-Lyon）的xZn/64 Zn同位素比，其中x表示66或68。
Analysis of naturally occurring isotopic variations is a promising tool for investigating Zn transport and cycling in geological and biological settings. Here, we present the recently installed double-spike (DS) technique at the MAGIC laboratories at Imperial College London. The procedure improves on previous published DS methods in terms of ease of measurement and precisions obtained. The analytical method involves addition of a 64Zn–67Zn double-spike to the samples prior to digestion, separation of Zn from the sample matrix by ion exchange chromatography, and isotopic analysis by multiple-collector inductively coupled plasma mass spectrometry. The accuracy and reproducibility of the method were validated by analyses of several in-house and international elemental reference materials. Multiple analyses of pure Zn standard solutions consistently yielded a reproducibility of about ±0.05‰ (2 SD) for δ66Zn, and comparable precisions were obtained for analyses of geological and biological materials. Highly fractionated Zn standards analyzed by DS and standard sample bracketing yield slightly varying results, which probably originate from repetitive fractionation events during manufacture of the standards. However, the δ66Zn values (all reported relative to JMC Lyon Zn) for two less fractionated in-house Zn standard solutions, Imperial Zn (0.10 ± 0.08‰: 2 SD) and London Zn (0.08 ± 0.04‰), are within uncertainties to data reported with different mass spectrometric techniques and instruments. Two standard reference materials, blend ore BCR 027 and ryegrass BCR 281, were also measured, and the δ66Zn were found to be 0.25 ± 0.06‰ (2 SD) and 0.40 ± 0.09‰, respectively. Taken together, these standard measurements ascertain that the double-spike methodology is suitable for accurate and precise Zn isotope analyses of a wide range of natural samples. The newly installed technique was consequently applied to soil samples and soil leachates to investigate the isotopic signature of plant available Zn. We find that the isotopic composition is heavier than the residual, indicating the presence of loosely bound Zn deposited by atmospheric pollution, which is readily available to plants. Zinc isotope ratio pools of bulk soil and the associated acid leach (estimated plant available pool) as measured by double-spike MC-ICPMS. δxZnLyon-JMC=(Rsample/RJMC-Lyon -1)x103, where Rsample and RJMC-Lyon denote the xZn/64Zn isotope ratio of the sample and standard (JMC-Lyon), respectively, and where x denotes either 66 or 68.
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影响因子：
3.4
作者：
Archer, C;Vance, D
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通讯作者：
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