Characterization of a dwarf gene in Brassica rapa, including the identification of a candidate gene
Characterization of a dwarf gene in Brassica rapa, including the identification of a candidate gene
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DOI：
10.1007/s00122-003-1551-2
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发表时间：
2004-05-01
影响因子：
5.4
通讯作者：
Osborn, TC
中科院分区：
文献类型：
--
作者：
Muangprom, A;Osborn, TC
矮秆基因对提高几种作物的可收获产量有价值，并且可能在油菜中有用。我们评估了一个矮秆基因，dwf 2，从白菜，以确定其表型效应和遗传特性。dwf 2突变体对外源GA（3）不敏感，表明该突变体不是赤霉素生物合成途径的突变体。矮秆表型由一个半显性等位基因控制。dwf 2纯合或杂合的近等基因系与高大野生型系相比，株高分别降低了47.4%或30.0%，这种降低是由于节间长度和节数减少所致。dwf 2纯合和杂合株系的一级分枝数与野生型株系相同或显著高于野生型株系，但开花时间没有差异。DWF 2基因定位于连锁群R6的底部，在与拟南芥2号染色体顶部具有同源性的区域中。与A. thaliana表明它是RGA（ga 1 -3的阻遏物）的同系物，RGA是小麦“绿色革命”基因的同系物。该矮秆基因可用于深入研究赤霉素途径，并减少杂交油菜品种的倒伏问题。
Dwarf genes have been valuable for improving harvestable yield of several crop plants and may be useful in oilseed Brassica. We evaluated a dwarf gene, dwf2, from Brassica rapa in order to determine its phenotypic effects and genetic characteristics. The dwf2 mutant was insensitive to exogenous GA(3) for both plant height and flowering time, suggesting that it is not a mutation in the gibberellin biosynthesis pathway. The dwarf phenotype was controlled by a semidominant allele at a single locus. Near-isogenic lines that were homozygous or heterozygous for dwf2 had 47.4% or 30.0% reduction in plant height, respectively, compared to the tall wild-type line, and the reduction was due to reduced internode length and number of nodes. The dwf2 homozygous and heterozygous lines had the same or significantly higher numbers of primary branches than the wild-type line, but did not differ in flowering time. The DWF2 gene was mapped to the bottom of linkage group R6, in a region having homology to the top of Arabidopsis thaliana chromosome 2. The map position of DWF2 in comparison to markers in A. thaliana suggests it is a homolog of RGA (repressor of ga1-3), which is a homolog of the wheat "Green Revolution" gene. This dwarf gene could be used to gain more insight on the gibberellin pathway and to reduce lodging problems in hybrid oilseed Brassica cultivars.