Characterization of the proteins present in the mucus of the flatworm Artioposthia triangulata (Dendy)
Characterization of the proteins present in the mucus of the flatworm Artioposthia triangulata (Dendy)
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DOI：
10.1016/s0305-0491(97)00328-3
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发表时间：
1998-02-01
影响因子：
2.2
通讯作者：
Walker, B
中科院分区：
文献类型：
--
作者：
McGee, C;Wisdom, GB;Walker, B
与其他陆生扁虫一样，由Artioposthia triangulata产生的粘液可能具有许多功能，包括保护免受环境因素和捕食者的影响，并为扁虫提供运动和粘附的润滑。没有以前的工作已经进行了表征的蛋白质存在于粘液的A。本研究是对三角形虫粘液的初步研究。通过SDS-聚丙烯酰胺凝胶电泳、生物素化肽亲和探针和API ZYM酶检测试剂盒分析粘液。结果显示，粘液中存在至少40种多肽，并且用生物素化探针的进一步研究将其中一种表征为胰凝乳蛋白酶样丝氨酸蛋白酶。(C)1998年爱思唯尔科学公司
In common with other terrestrial flatworms, the mucus produced by Artioposthia triangulata may have a number of functions, including protection from environmental factors and from predators, and it provides the flatworm with lubrication for movement and adhesion. No previous work has been carried out on the characterization of proteins present in the mucus of A. triangulata and this study was a preliminary investigation of the mucus. Mucus was analysed by SDS-polyacrylamide gel electrophoresis, biotinylated peptide affinity probes and the API ZYM enzyme detection kit. Results have revealed the presence of at least 40 polypeptides in the mucus and further studies with biotinylated probes have characterised one of them as a chymotrypsin-like serine protease. (C) 1998 Elsevier Science Inc.