Forces for morphogenesis investigated with laser microsurgery and quantitative modeling
Forces for morphogenesis investigated with laser microsurgery and quantitative modeling
复制标题
DOI：
10.1126/science.1079552
复制
发表时间：
2003-04-04
期刊：
影响因子：
56.9
通讯作者：
Edwards, GS
中科院分区：
文献类型：
--
作者：
Hutson, MS;Tokutake, Y;Edwards, GS
我们研究了果蝇发育的遗传程序与形态发生之间的联系。我们专注于背侧闭合，这是一个强大的发育和伤口愈合模型系统。我们发现，大部分的进展走向关闭是由收缩性在超细胞的“荷包”和浆膜，而粘附介导的拉链协调的荷包产生的力量，是必不可少的，只有在最后阶段。我们应用定量建模来表明，这些在不同细胞中产生的力在空间上是协调的，在时间上是同步的。野生型和突变体表型的建模是预测性的，虽然关闭在肌球蛋白突变体最终失败时，细胞片撕裂自己分开，我们的分析表明，β（PS）整联蛋白具有较早的，重要的作用拉链。
We investigated the forces that connect the genetic program of development to morphogenesis in Drosophila. We focused on dorsal closure, a powerful model system for development and wound healing. We found that the bulk of progress toward closure is driven by contractility in supracellular "purse strings" and in the amnioserosa, whereas adhesion-mediated zipping coordinates the forces produced by the purse strings and is essential only for the end stages. We applied quantitative modeling to show that these forces, generated in distinct cells, are coordinated in space and synchronized in time. Modeling of wild-type and mutant phenotypes is predictive; although closure in myospheroid mutants ultimately fails when the cell sheets rip themselves apart, our analysis indicates that beta(PS) integrin has an earlier, important role in zipping.