LOCAL REGENERATION IN THE RETINA OF THE GOLDFISH
LOCAL REGENERATION IN THE RETINA OF THE GOLDFISH
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DOI：
10.1002/neu.480230209
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发表时间：
1992-03-01
期刊：
影响因子：
--
通讯作者：
JONES, DD
中科院分区：
文献类型：
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作者：
HITCHCOCK, PF;MYHR, KJL;JONES, DD
我们研究了金鱼视网膜的再生，作为中枢神经系统再生神经发生的模型。 使用经巩膜手术方法，我们切除了一小块视网膜，这些视网膜在几周内被再生所取代。研究了三组动物的受损视网膜，分别表征再生过程中视网膜和周围组织的质变、伴随的细胞增殖以及再生和完整视网膜之间的定量关系。使用光学显微镜标准方法制备的视网膜进行定性和定量分析。在一组动物中计算再生和完整视网膜神经元的平面密度，其中正常平面密度范围从高到低。通过在不同存活时间眼内注射 5-溴-2'-脱氧尿苷 (BUdr) 以标记增殖细胞并处理视网膜切片进行 BUdr 免疫细胞化学研究来研究细胞增殖。定性分析显示，手术在现有视网膜上造成了一个缺口，并在接下来的几周内被新的视网膜取代。 BUdr 标记实验表明，切除的视网膜被新神经元的再生所取代。神经上皮样细胞聚集在伤口边缘并向心迁移，在旧视网膜上添加新的视网膜。定量分析表明，再生神经元的平面密度接近完整神经元的平面密度。
We have studied regeneration of the retina in the goldfish as a model of regenerative neurogenesis in the central nervous system. Using a transscleral surgical approach, we excised small patches of retina that were replaced over several weeks by regeneration. Lesioned retinas from three groups of animals were studied to characterize, respectively, the qualitative changes of the retina and surrounding tissues during regeneration, the concomitant cellular proliferation, and the quantitative relationship between regenerated and intact retina. The qualitative and quantitative analyses were done on retinas prepared using standard methods for light microscopy. The planimetric density of regenerated and intact retinal neurons was computed in a group of animals in which the normal planimetric density ranged from high to low. Cell proliferation was investigated by making intraocular injections of 5-bromo-2'-deoxyuridine (BUdr) at various survival times to label proliferating cells and processing retinal sections for BUdr immunocytochemistry. The qualitative analysis showed that the surgery created a gap in the existing retina that was replaced with new retina over the subsequent weeks. The BUdr-labeling experiments demonstrated that the excised retina was replaced by regeneration of new neurons. Neuroepithial-like cells clustered on the wound margin and migrated centripetally, appositionally adding new retina to the old. The quantitative analysis showed that the planimetric density of the regenerated neurons approximated that of the intact ones.