Origin of Dissolved-phase Hyperpolarized 129Xe Signal in the Mouse Chest Based on Experimental Evidence from Extensive Magnetic Resonance Measurements
Origin of Dissolved-phase Hyperpolarized 129Xe Signal in the Mouse Chest Based on Experimental Evidence from Extensive Magnetic Resonance Measurements
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DOI：
10.2463/mrms.10.149
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发表时间：
2011-01-01
影响因子：
3
通讯作者：
Fujiwara, Hideaki
中科院分区：
文献类型：
--
作者：
Narazaki, Michiko;Kimura, Atsuomi;Fujiwara, Hideaki
使用超极化（HP）氙-129气体作为成像介质的肺部研究必须关注溶解相信号，以充分利用氙对生物组织（包括血液）的特性亲和力。然而，这些信号的光谱模式在小鼠和其他动物（包括大鼠、犬和人类）之间是不同的。由于在小鼠中的溶解相研究报道较少，因此谱图的归属成为HP MRS-129磁共振波谱（MRS）和MR成像的重要研究课题，以使其得到更广泛的应用。我们进行了MRS，包括磁化转移实验，和MR成像研究，以确认小鼠离体和体内的溶解相信号的来源，并获得证据，分配溶解相信号在192 ppm的心外膜脂肪，196 ppm的肺实质，和200 ppm的血液。这些结果首次显示了氙在血浆和红细胞之间快速交换的可能性。
Pulmonary study using hyperpolarized (HP) Xe-129 gas as an imaging medium must focus on dissolved-phase signals to make the most of the characteristic affinity of xenon for biological tissues, including blood. However, the spectral pattern of these signals differs between mice and other animals, including rats, canines, and humans. Dissolved-phase study has been reported only scarcely in mice, so spectral assignment has been an important subject for HP Xe-129 magnetic resonance (MR) spectroscopy (MRS) and MR imaging for its wider application. We performed MRS, including magnetization transfer experiments, and MR imaging studies to confirm the origin of dissolved-phase signals of mice ex vivo and in vivo and obtained evidence to assign dissolved-phase signals at 192 ppm for epicardial fat, 196 ppm for lung parenchyma, and 200 ppm for blood. These results were the first to show the possibility of fast exchange of xenon between plasma and red blood cells.