Standardization of the immunocytochemical detection of cancer cells in BM and blood: I. establishment of objective criteria for the evaluation of immunostained cells
Standardization of the immunocytochemical detection of cancer cells in BM and blood: I. establishment of objective criteria for the evaluation of immunostained cells
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DOI：
10.1080/0032472031000141283
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发表时间：
1999-01-01
期刊：
影响因子：
4.5
通讯作者：
Pantel, K
中科院分区：
文献类型：
--
作者：
Borgen, E;Naume, B;Pantel, K
背景检测来自癌症患者的骨髓中分离的肿瘤细胞（TC）可以预测未来的复发。各种分子和免疫细胞化学 (ICC) 方法已用于检测这些细胞，这些细胞的存在频率极低，为 10(-5)-10(-6)。这些技术的特异性和灵敏度可能差异很大。1996 年，Europez ISHAGE 工作组成立，旨在标准化和优化用于检测微小残留病的程序。我们试图制定客观标准来评估免疫细胞化学可识别的癌细胞。方法进行了实验室间环形实验来比较不同实验室之间微转移阳性事件的筛查和检测。不一致的结果促使lu建立了适用于识别免疫染色微转移TC的形态学标准的共同共识。结果基于此共识评估，我们建议将染色元素分为三组：（1）'TC'S显示上皮TC性质的特征标志，由明显增大的细胞核或大于或等于2的簇定义。 免疫阳性细胞。 (2) '可能的 TCS 代表造血细胞 (TIC) 和 TC 之间的形态重叠，缺乏 TC 性质的特征标志，但不表现出 HC 的明显形态特征。如果同种型特异性、不相关的抗体的对照染色呈阴性，则这些细胞被视为 TC。 (3)“TC阴性”细胞被定义为“假阳性”HC、皮肤鳞状上皮细胞和伪影。讨论所提出的免疫染色事件分类是开发用于检测BM或血液中隐匿性微转移TC的标准化免疫细胞化学测定的第一步。
BackgroundDetection of isolated tumor cells (TC) in BM from carinoma patients can predict future relapse. Various molecular and immunocytochemical (ICC) methods have been used to detect these cells, which are present at extremely low frequencies of 10(-5)-10(-6) The specificity and sensitivity of these techniques may vary widely In 1996, a Europeanz ISHAGE Working Group was founded to standardize and optimize procedure used for the detection of minimal residual disease. We have attempted to develop objective criteria for the evaluation of immunocytochemically, identifiable cancer cells.MethodsAn interlaboratory ring experiment was performed to compare the screening and detection of micrometastasis-positive events between different laboratories. The discrepant results induced lu to establish a common consensus on morphological criteria applicable to the identification of immunostained micrometastatic TC.ResultsBased on this consensus evaluation, we propose a classification of stained elements into three groups: (1) 'TC'S show pathognomonic signs of epithelial TC-nature, as defined by a clearly enlarged nucleus or clusters of greater than or equal to 2 immunopositive cells. (2) 'Probable TCS represent morphological overlap between hematopoietic cells (TIC) and TC which lack pathognomonic signs of TC-nature, but do not exhibit clear morphological features of HC. These cells are considered as TC if control staining with an isotype-specific, unrelated Ab is negative. (3) 'TC-negative' cells are defined as 'false positive' HC, skin squamous epithelial cells and artefacts.DiscussionThe proposed classification of immunostained events is a first step towards the development of standardized immunocytochemical assays for the detection of occult micrometastatic TC in BM or blood.