Diversity and Global Distribution of IncL/M Plasmids Enabling Horizontal Dissemination of β-Lactam Resistance Genes among the Enterobacteriaceae.
Diversity and Global Distribution of IncL/M Plasmids Enabling Horizontal Dissemination of β-Lactam Resistance Genes among the Enterobacteriaceae.
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DOI：
10.1155/2015/414681
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发表时间：
2015
影响因子：
--
通讯作者：
Bartosik D
中科院分区：
文献类型：
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作者：
Adamczuk M;Zaleski P;Dziewit L;Wolinowska R;Nieckarz M;Wawrzyniak P;Kieryl P;Plucienniczak A;Bartosik D
抗生素耐药决定簇通常与质粒和其他移动的遗传元件相关，这简化了它们的水平传播。发现几组质粒（包括IncL/M不相容组的复制子）在编码β-内酰胺酶的耐药基因的传播中起重要作用。IncL/M质粒是大的、广泛的宿主范围的和自我传播的复制子。我们已经确定并表征了该组的两个新成员：pARM 26（从废水处理厂的活性污泥中分离出的细菌）和pIGT 15（来自大肠杆菌的临床菌株）。这促使对编码β-内酰胺酶的IncL/M质粒的所有可用序列进行详细的比较分析。这些质粒的核心基因组由20个具有保守同线性的基因组成。这些核心基因的系统发育分析允许将质粒聚类成四个独立的组，这反映了它们的抗生素耐药性谱。对IncL/M质粒的电泳分析表明，它们最常发现于源自地中海地区和西欧的肠杆菌科细菌中，即使在没有直接抗生素选择压力的情况下，它们也能够在各种生态环境中持续存在。
Antibiotic resistance determinants are frequently associated with plasmids and other mobile genetic elements, which simplifies their horizontal transmission. Several groups of plasmids (including replicons of the IncL/M incompatibility group) were found to play an important role in the dissemination of resistance genes encoding β-lactamases. The IncL/M plasmids are large, broad host range, and self-transmissible replicons. We have identified and characterized two novel members of this group: pARM26 (isolated from bacteria inhabiting activated sludge from a wastewater treatment plant) and pIGT15 (originating from a clinical strain of Escherichia coli). This instigated a detailed comparative analysis of all available sequences of IncL/M plasmids encoding β-lactamases. The core genome of these plasmids is comprised of 20 genes with conserved synteny. Phylogenetic analyses of these core genes allowed clustering of the plasmids into four separate groups, which reflect their antibiotic resistance profiles. Examination of the biogeography of the IncL/M plasmids revealed that they are most frequently found in bacteria of the family Enterobacteriaceae originating from the Mediterranean region and Western Europe and that they are able to persist in various ecological niches even in the absence of direct antibiotic selection pressure.