Novel motifs in amino acid permease genes from Leishmania
Novel motifs in amino acid permease genes from Leishmania
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DOI：
10.1016/j.bbrc.2004.09.212
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发表时间：
2004-12-03
影响因子：
3.1
通讯作者：
Zilberstein, D
中科院分区：
文献类型：
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作者：
Akerman, M;Shaked-Mishan, P;Zilberstein, D
从原生动物寄生虫杜氏利什曼原虫(Leishmania donovani，AAPLDs)中克隆了8个氨基酸渗透酶基因，并对其进行了测序，结果表明该基因在前鞭毛体中表达。其中7个属于氨基酸转运蛋白-1，1个属于氨基酸多氨基胆碱超家族。利用这些序列以及来自所有王国的已知和表征的氨基酸渗透酶基因，建立了一个训练集，并使用模因模体发现工具来搜索模体。本研究发现了两个利什曼原虫属特有的基序，四个锥虫科的基序，以及锥虫科和哺乳动物系统A1和N之间常见的单一基序。有趣的是，这些基序大多聚集在50-60个氨基酸的两个区域。BLAST搜索分析表明，杜氏钩端螺旋体与布氏锥虫的氨基酸渗透率关系密切。这项工作的结果描述了寄生原生动物中第一个氨基酸渗透酶基因的克隆，并有助于了解这些生物中氨基酸渗透酶的进化。此外，在这些蛋白质中识别特定属的基序可能有助于更好地了解寄主内的寄生虫生理。(C)2004 Elsevier Inc.保留所有权利。
Eight amino acid permease genes from the protozoan parasite Leishmania donovani (AAPLDs) were cloned, sequenced, and shown to be expressed in promastigotes. Seven of these belong to the amino acid transporter-1 and one to the amino acid polyamino-choline superfamilies. Using these sequences as well as known and characterized amino acid permease genes from all kingdom's, a training set was established and used to search for motifs, using the MEME motif discovery tool. This study revealed two motifs that are specific to the genus Leishmania, four to the family trypanosomatidae, and a single motif that is common between trypanosomatidae and mammalian systems A1 and N. Interestingly, most of these motifs are clustered in two regions of 50-60 amino acids. Blast search analyses indicated a close relationship between the L. donovani and Trypanosoma brucei amino acid permeases. The results of this work describe the cloning of the first amino acid permease genes in parasitic protozoa and contribute to the understanding of amino acid permease evolution in these organisms. Furthermore, the identification of genus-specific motifs in these.proteins might be useful to better understand parasite physiology within its hosts. (C) 2004 Elsevier Inc. All rights reserved.