Silicone 3D Printing: Process Optimization, Product Biocompatibility, and Reliability of Silicone Meniscus Implants
Silicone 3D Printing: Process Optimization, Product Biocompatibility, and Reliability of Silicone Meniscus Implants
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DOI：
10.1089/3dp.2018.0226
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发表时间：
2019-12-01
影响因子：
3.1
通讯作者：
Yeong, Wai Yee
中科院分区：
文献类型：
--
作者：
Luis, Eric;Pan, Houwen Matthew;Yeong, Wai Yee
采用新型定制3D硅胶打印机和两组分Ecoflex有机硅树脂，采用热固化挤出法3D打印标准形状的硅胶片和不规则形状的半月板结构。本文分为三个部分：(1)3D打印参数对3D打印硅胶尺寸精度和力学性能的影响研究；(2)按照ASTM D575标准对3D打印硅胶在单调和循环压缩载荷下的可靠性和失效分析；(3)按照ISO 10993-12标准提取方法对3D打印硅胶在不同提取时间和提取体积/表面积比下的细胞毒性。通过回归分析和方差分析发现，长度和宽度的尺寸精度对喷嘴直径和床层温度均敏感，而高度仅对床层温度敏感。采用双参数Weibull概率分布模型和Weibull回归分析对失败结果进行分析，发现各组的Weibull模量均大于1，说明Ecoflex 30和50半月板植入物失败率随时间增加而增加。细胞增殖定量实验结果显示，所有样品的差异在统计学上不显著，表明印刷硅胶具有低细胞毒性和良好的生物相容性。
A novel custom-made 3D silicone printer and two-part Ecoflex silicone resins were used to 3D-print standard-shaped silicone coupon and irregular-shaped meniscus structures via a heat-cured extrusion-based method. This article is segmented into three parts: (1) study on the effect of 3D printing parameters on dimensional accuracy and mechanical properties of 3D-printed silicone, (2) reliability and failure analysis of 3D-printed silicone according to ASTM D575 standards under monotonic and cyclic compressive loading, and (3) cytotoxicity of 3D-printed silicone by extraction method according to ISO 10993-12 for different extraction time and extract volume/surface area ratios. Based on analysis using regression method and analysis of variance, we found that the dimensional accuracy of lengths and widths is sensitive to both nozzle diameters and bed temperatures (BTs), while the height is only sensitive to BTs. Failure results were analyzed using the two-parameter Weibull probability distribution model and Weibull regression analysis and revealed that the Weibull modulus had a value greater than 1 in all groups, indicating an increasing failure rate with time for Ecoflex 30 and 50 meniscus implants. Results from quantitative cell proliferative assay exhibit statistically insignificant differences for all samples, pointing to the low cytotoxicity and excellent biocompatibility of printed silicone.