Screening and Antiviral Analysis of Phages That Display Peptides with an Affinity to Subunit C of Porcine Aminopeptidase
Screening and Antiviral Analysis of Phages That Display Peptides with an Affinity to Subunit C of Porcine Aminopeptidase
复制标题
展示与猪氨肽酶 C 亚基有亲和力的肽的噬菌体的筛选和抗病毒分析
DOI：
10.1089/mab.2013.0038
复制
发表时间：
2013-10-01
影响因子：
--
通讯作者：
Sun, Dongbo
中科院分区：
文献类型：
--
作者：
Guo, Donghua;Zhu, Qinghe;Sun, Dongbo
以纯化的重组猪氨肽酶N（rpAPN-C）蛋白C亚基为固定化靶点，从噬菌体随机肽库中筛选rpAPN-C的潜在配体。经过5轮生物淘选，5个噬菌体克隆显示出与rpAPN-C的特异性结合亲和力。在3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四氮唑（MTT）试验中，含有HDAISWTHYHPW肽序列的噬菌体克隆PM 1在猪睾丸细胞中对TGEV感染具有保护作用。因此，HDAISWTHYHPW肽序列具有作为抗TGEV感染的小分子治疗剂的潜在用途。
The purified C subunit of the recombinant porcine aminopeptidase N (rpAPN-C) protein was used as an immobilized target to screen potential ligands against rpAPN-C from a 12-mer phage display random peptide library. After five rounds of biopanning, five phage clones showed specific binding affinities to rpAPN-C. In 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assays, the phage clone PM1, which contained the HDAISWTHYHPW peptide sequence, had a protective effect against TGEV infection in swine testis cells. Therefore, the HDAISWTHYHPW peptide sequence has a potential use as a small molecular therapeutic agent against TGEV infection.