Cytokine loaded layer-by-layer ultrathin matrices to deliver single dermal papilla cells for spot-by-spot hair follicle regeneration
Cytokine loaded layer-by-layer ultrathin matrices to deliver single dermal papilla cells for spot-by-spot hair follicle regeneration
复制标题
加载细胞因子的逐层超薄基质可输送单个真皮乳头细胞，实现逐点毛囊再生
DOI：
10.1039/c5tb02265g
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发表时间：
2016-01-01
影响因子：
7
通讯作者：
Xing, Malcolm
中科院分区：
文献类型：
--
作者：
Lin, Bo-jie;Wang, Jin;Xing, Malcolm
毛乳头细胞(DPC)是一种位于毛囊中的高度特化的间充质细胞。由于在毛发-毛囊形态发生和毛发周期的上皮-间充质相互作用中起主要作用，DPC已成为治疗脱发患者毛发再生的诱人细胞来源。然而，在体外培养过程中，DPC往往会失去其功能。因此，显然需要开发一种微环境，以概括DPC的本地环境中的相互作用。由于在细胞表面采用生物相容材料的逐层纳米涂层具有可调节的负载和释放特性，因此可以为细胞功能的调节提供一个重塑的微环境。在这里，我们开发了一种LBL自组装技术来单个DPC来创建纳米级的超薄细胞外基质(ECM)。通过Western印迹和β-连环素、碱性磷酸酶、α-平滑肌肌动蛋白基因的表达，我们研究了基于LBL的单细胞包裹不会影响DPC的活性、形态、增殖和内在特性。然后，我们将成纤维细胞生长因子-2(成纤维细胞生长因子-2)引入LBL纳米结构，以调节DPC的功能。最后，我们进行了体内毛发重建试验，使用LBL包裹的DPC和新鲜分离的表皮细胞(EPC)相结合，发现这种策略可以治疗脱发。
The dermal papilla cell (DPC) is a type of highly specialized mesenchymal cells located in hair follicles (HF). Due to the primary role in the epithelial-mesenchymal interaction that enables hair-follicle morphogenesis and hair cycling, DPC has become an attractive cell source for hair regeneration to treat alopecia patients. However, DPCs tend to lose their function during in vitro culture. Hence, there exists a clear need to develop a microenvironment that can recapitulate the interactions within the native milieu of DPCs. Since layer-by-layer (LBL) nano-coating with biocompatible materials on the cell surface displays the versatility with tunable loading and release properties, which can provide a remodeled microenvironment for regulating cell function. Here, we developed a LBL self-assembly technique to single DPCs to create a nano-scale ultrathin extracellular matrix (ECM). We studied that the single cell-based LBL-encapsulation would not impact the viability, morphology, proliferation and intrinsic properties of DPCs using Western blot and mRNA expressions of beta-catenin, ALP and alpha-SMA. We then included fibroblast growth factor-2 (FGF-2) into the LBL nano-structure to regulate the DPC function. Finally, we performed in vivo hair reconstitution assays using LBL-encapsulated DPCs combined with freshly isolated epidermal cells (EPCs) and found this strategy can treat hair loss.