Lifecycle, culture, and maintenance of the emerging cephalopod models Euprymna berryi and Euprymna morsei
Lifecycle, culture, and maintenance of the emerging cephalopod models Euprymna berryi and Euprymna morsei
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DOI：
10.3389/fmars.2022.1039775
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发表时间：
2022-12-02
影响因子：
3.7
通讯作者：
Rokhsar, Daniel
中科院分区：
文献类型：
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作者：
Jolly, Jeffrey;Hasegawa, Yuko;Rokhsar, Daniel
头足类动物研究仍然受到限制，因为无法在实验室条件下对物种进行多代养殖，以便在生命周期的各个阶段都能持续获取动物。在此，我们描述了一种针对两种新兴头足类动物模型的多代实验室养殖系统：蜂鸟鱿鱼或贝氏短尾鱿鱼（Euprymna berryi Sasaki，1929）以及莫氏短尾鱿鱼（Euprymna morsei Verrill，1881），它们主要分布在日本本土沿海。贝氏短尾鱿鱼的野生成年个体产卵并被养殖到第三代，莫氏短尾鱿鱼的野生成年个体产卵并被养殖到第二代，养殖是在20℃的封闭系统中进行的。我们报告了圈养第一代中30个个体的生长和存活数据。贝氏短尾鱿鱼和莫氏短尾鱿鱼在孵化后的前90天和60天分别呈指数增长。贝氏短尾鱿鱼和莫氏短尾鱿鱼首次产卵时的存活率分别为90%和77%。贝氏短尾鱿鱼和莫氏短尾鱿鱼的雌性分别在孵化后112天和71天产卵。我们描述了每个物种的生活史以及如何区分性别。我们讨论了头足类动物养殖面临的挑战以及养殖这些物种如何解决这些问题。
Cephalopod research remains limited by the inability to culture species under laboratory conditions for multiple generations to provide continuous access to animals at all stages of the life cycle. Here, we describe a multi-generational laboratory culture system for two emerging cephalopod models: the hummingbird or Berry's bobtail squid, Euprymna berryi Sasaki, 1929, and Morse's bobtail squid, Euprymna morsei Verrill, 1881, which are primarily found off mainland Japan. E. berryi wild adults were spawned and raised to the third filial generation, and E. morsei wild adults were spawned and raised to the second filial generation in a closed system at 20 degrees C. We report growth and survivorship data for a cohort of 30 individuals across the first generation raised in captivity. E. berryi and E. morsei grew exponentially during the first 90 and 60 days post-hatching, respectively. Survivorship at the first spawning event for E. berryi and E. morsei was 90% and 77%. E. berryi and E. morsei females spawned after days 112 and 71 days post-hatching, respectively. We describe the life history of each species and how to distinguish sexes. We discuss the challenges of cephalopod culture and how culturing these species address those problems.