Expression of estrogen receptor α and β genes in the mediobasal hypothalamus, pituitary and ovary during the canine estrous cycle
Expression of estrogen receptor α and β genes in the mediobasal hypothalamus, pituitary and ovary during the canine estrous cycle
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DOI：
10.1016/s0304-3940(03)00639-6
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发表时间：
2003-08-21
影响因子：
2.5
通讯作者：
Inaba, T
中科院分区：
文献类型：
--
作者：
Hatoya, S;Torii, R;Inaba, T
在发情周期的各个阶段，测量比格犬母犬的下丘脑内侧基底、垂体前叶和卵巢中的雌激素受体α (ERα) 和 ERP mRNA 水平。通过聚合酶链反应分析，我们在所有组织样本中检测到了 ERP 基因转录本。下丘脑和垂体 ERα 和 β mRNA 的水平从动情中期到动情前期增加，此后下降。在卵巢中，ERa mRNA水平从发情前期到发情间期增加，并与血浆孕酮水平呈正相关（r = 0.62，P < 0.01），而ERP mRNA水平从动情中期到发情前期增加，并与血浆雌二醇-17β水平呈正相关（r = 0.73，P < 0.001）。这些结果表明，下丘脑和垂体 ERα 和 P mRNA 的增加与不发情的终止相关，而卵巢 ERα 和 β mRNA 的增加可能与卵泡或黄体发育的启动有关。 (C) 2003 Elsevier Science Ireland Ltd. 保留所有权利。
Estrogen receptor alpha (ERalpha) and ERP mRNA levels were measured in the mediobasal hypothalamus, the anterior pituitary and the ovary of beagle bitches at various stages of the estrous cycle. With polymerase chain reaction analysis we detected ERP gene transcripts in all tissue samples. The levels of hypothalamic and pituitary ERalpha and beta mRNAs increased from mid anestrus to proestrus and declined thereafter. In the ovary, ERa mRNA levels increased from proestrus to diestrus and were positively correlated with plasma progesterone levels (r = 0.62, P < 0.01), whereas ERP mRNA levels increased from mid anestrus to proestrus and were positively correlated with plasma estradiol-17beta levels (r = 0.73, P < 0.001). These results suggest that the rise in hypothalamic and pituitary ERalpha and P mRNAs is associated with termination of anestrus, and that increases in ovarian ERalpha and beta mRNAs may be involved in initiating development of the follicle or corpora lutea. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.