Enhanced antibacterial effect against Enterococcus faecalis by silver ions plus Triton X-100 with low concentrations and cytotoxicity.
Enhanced antibacterial effect against Enterococcus faecalis by silver ions plus Triton X-100 with low concentrations and cytotoxicity.
复制标题
银离子加低浓度的 Triton X-100 增强对粪肠球菌的抗菌作用，并具有细胞毒性。
DOI：
10.1007/s42770-021-00643-8
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Wei Fan
中科院分区：
文献类型：
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作者：
Qing Sun;Mengting Duan;Bing Fan;Wei Fan
粪肠球菌（E. faecalis）通常被认为是继发性和持续性根管感染的罪魁祸首之一。由于抗生素耐药性已成为全球性问题，为了减少抗生素的使用，金属离子最近被广泛使用作为替代品。银离子（Ag+）已被证明是一种强杀菌剂，但具有高细胞毒性和变色性。 Triton X-100（TX-100）与Ag+首次联合用作临床根管内药物，获得增强的抗菌作用和低细胞毒性。 TX-100 + Ag+ 对牙本质上的浮游菌和生物膜粪肠球菌的协同抗菌作用进行了测试。并在MC3T3-E1细胞上测试细胞毒性。结果证实，加入 TX-100 后，对浮游和生物膜驻留粪肠球菌的抗菌活性显着提高。 TX-100 和 Ag+ 混合物表现出与 2% 氯己定 (CHX) 相似的抑制作用，而细胞毒性远低于 2% CHX (p < 0.05)。总之，TX-100+Ag+混合物可能被开发成一种新的有效的根管内药物，如2%CHX。
Enterococcus faecalis (E. faecalis) is commonly considered to be one of chief culprits of secondary and persistent root canal infections. As antibiotic resistance has become a global issue, in order to reduce the use of antibiotics, metal ions have recently been widely used as an alternative. Silver ions (Ag+) have been proved to be a strong bactericide but with high cytotoxicity and discoloration property. Triton X-100 (TX-100) and Ag+ were co-used for the first time as a clinical intracanal medication to obtain both enhanced antibacterial effect and low cytotoxicity. The synergistic antibacterial effect of TX-100 + Ag+ was tested on both planktonic and biofilm-resident E. faecalis on dentine. And the cytotoxicity was tested on MC3T3-E1 cells. Results confirmed the antibacterial activity against both planktonic and biofilm-resident E. faecalis was dramatically improved after TX-100 incorporation. TX-100 and Ag+ mixture demonstrated a similar inhibitory effect as the 2% chlorhexidine (CHX), while the cytotoxicity was much lower than 2% CHX (p < 0.05). In conclusion, TX-100 + Ag+ mixture might be developed into a new effective intracanal medication as the 2% CHX.