Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses
Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses
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DOI：
10.1084/jem.20201181
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发表时间：
2020-11-01
影响因子：
15.3
通讯作者：
Bieniasz, Paul D.
中科院分区：
文献类型：
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作者：
Schmidt, Fabian;Weisblum, Yiska;Bieniasz, Paul D.
SARS-CoV-2的出现和随后爆发的COVID-19疾病的流行产生了对快速方便地测量SARS-CoV-2特异性抗体的抗病毒活性的测定的需求。在这里，我们描述了一个收集的方法的基础上，SARS-CoV-2尖峰假型，单周期，复制缺陷型人类免疫缺陷病毒1型（HIV-1），水泡性口炎病毒（VSV），以及复制能力VSV/SARS-CoV-2嵌合病毒。虽然每种替代病毒在检测中和活性的灵敏度方面表现出细微的差异，但使用每种病毒测量的恢复期血浆和人单克隆抗体的中和活性与使用真实SARS-CoV-2中和试验测量的中和活性定量相关。本文所述的测定适用于高通量，并且是评价由疫苗接种或先前SARS-CoV-2感染赋予的血清学免疫以及恢复期血浆或人单克隆抗体的效力的有用工具。
The emergence of SARS-CoV-2 and the ensuing explosive epidemic of COVID-19 disease has generated a need for assays to rapidly and conveniently measure the antiviral activity of SARS-CoV-2-specific antibodies. Here, we describe a collection of approaches based on SARS-CoV-2 spike-pseudotyped, single-cycle, replication-defective human immunodeficiency virus type-1 (HIV-1), and vesicular stomatitis virus (VSV), as well as a replication-competent VSV/SARS-CoV-2 chimeric virus. While each surrogate virus exhibited subtle differences in the sensitivity with which neutralizing activity was detected, the neutralizing activity of both convalescent plasma and human monoclonal antibodies measured using each virus correlated quantitatively with neutralizing activity measured using an authentic SARS-CoV-2 neutralization assay. The assays described herein are adaptable to high throughput and are useful tools in the evaluation of serologic immunity conferred by vaccination or prior SARS-CoV-2 infection, as well as the potency of convalescent plasma or human monoclonal antibodies.