Genome-Wide Wheat 55K SNP-Based Mapping of Stripe Rust Resistance Loci in Wheat Cultivar Shaannong 33 and Their Alleles Frequencies in Current Chinese Wheat Cultivars and Breeding Lines
Genome-Wide Wheat 55K SNP-Based Mapping of Stripe Rust Resistance Loci in Wheat Cultivar Shaannong 33 and Their Alleles Frequencies in Current Chinese Wheat Cultivars and Breeding Lines
复制标题
基于全基因组小麦55K SNP的小麦品种陕农33条锈病抗性位点及其等位基因频率在中国现有小麦品种和育种系中的定位
DOI：
10.1094/pdis-07-20-1516-re
复制
发表时间：
2021-04-01
期刊：
影响因子：
4.5
通讯作者：
Wu, Jianhui
中科院分区：
文献类型：
--
作者：
Huang, Shuo;Liu, Shengjie;Wu, Jianhui
小麦品种陕农33（SN 33）自2009年推广以来，在田间对条锈病保持高度抗性。为了揭示条锈抗性的遗传结构，用两个条锈病原菌（条锈病菌）分离物（PST-Lab.1和PST-Lab.2）评价了Avocet S x SN 33杂交的161个重组自交系（RIL）的幼苗。在两个种植季节期间，在自然或人工接种的田间地点对RIL进行了评价。利用小麦55 K单核苷酸多态性芯片对RIL及其亲本进行基因分型。在1DS、2AS和3DS染色体上定位了3个苗期抗病基因组区域，在1BL、2AS、3DL和6 BS染色体上检测到4个成株抗性QTL。2AS基因定位于2NS易位系上，为Yr 17基因。在1BL和6 BS上鉴定的QTL可能分别对应于Yr 29和Yr 78。在3DL上的一个APR QTL解释了5.8%至12.2%的表型变异，可能是新的。利用2NS片段（Yr 17）、Yr 29、Yr 78和QYrsn.n-wafu-3DL对420份小麦品种和育种材料进行分子标记检测，结果表明，这些基因分别在11.4%、7.6%、14.8%和7.4%的材料中存在。这些基因与QTL间的互作均为加性互作，表明它们在提高抗条锈病育种材料中的潜在价值。此外，我们还鉴定了两个叶片坏死基因，Ne 1和Ne 2;然而，来自Avocet S × SN 33的F1植株存活，表明SN 33可能具有允许种子收获的Ne 1的另一个等位基因。
Wheat cultivar Shaannong 33 (SN33) has remained highly resistant to stripe rust in the field since its release in 2009. To unravel the genetic architecture of stripe rust resistance, seedlings of 161 recombinant inbred lines (RILs) from the cross Avocet S x SN33 were evaluated with two isolates (PST-Lab.1 and PST-Lab.2) of the stripe rust pathogen (Puccinia striiformis f. sp. tritici) in the greenhouse, and the RILs were evaluated in naturally or artificially inoculated field sites during two cropping seasons. The RILs and parents were genotyped with the wheat 55K single-nucleotide polymorphism array. Three genomic regions conferring seedling resistance were mapped on chromosomes 1DS, 2AS, and 3DS, and four consistent quantitative trait loci (QTL) for adult-plant resistance (APR) were detected on 1BL, 2AS, 3DL, and 6BS. The 2AS locus conferring all-stage resistance was identified as the resistant gene Yr17 located on 2NS translocation. The QTL identified on 1BL and 6BS likely correspond to Yr29 and Yr78, respectively. An APR QTL on 3DL explaining 5.8 to 12.2% of the phenotypic variation is likely to be new. Molecular marker detection assays with the 2NS segment (Yr17), Yr29, Yr78, and QYrsn.n-wafu-3DL on a panel of 420 current Chinese wheat cultivars and breeding lines indicated that these genes were present in 11.4, 7.6, 14.8, and 7.4% of entries, respectively. The interactions among these genes and QTL were additive, suggesting their potential value in enhancing stripe rust resistance breeding materials as observed in the resistant parent. In addition, we also identified two leaf necrosis genes, Ne1 and Ne2; however, the F-1 plants from cross Avocet S x SN33 survived, indicating that SN33 probably has another allele of Ne1 which allows seed to be harvested.