Molecular epidemiology of clinical and environmental isolates of the Cryptococcus neoformans species complex reveals a high genetic diversity and the presence of the molecular type VGII mating type a in Colombia
Molecular epidemiology of clinical and environmental isolates of the Cryptococcus neoformans species complex reveals a high genetic diversity and the presence of the molecular type VGII mating type a in Colombia
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DOI：
10.1111/j.1567-1364.2006.00055.x
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发表时间：
2006-06-01
影响因子：
3.2
通讯作者：
Castaneda, Elizabeth
中科院分区：
文献类型：
--
作者：
Escandon, Patricia;Sanchez, Adriana;Castaneda, Elizabeth
本研究的目的是调查在哥伦比亚的新型隐球菌物种复杂的临床和环境分离株的流行病学关系。目前的研究反映了1987年至2004年的数据。在哥伦比亚，血清型A和B最常从患者和环境中回收。在178株临床分离株中，91.1%为A型，8.4%为B型，0.5%为C型。247株环境菌株中，A型占44.2%，B型占42.6%，C型占13.2%。未分离到血清型D分离株。在哥伦比亚尚未发现AD血清型。用引物M13、（GACA）（4）和（GTG）（5）的PCR指纹图谱和URA 5基因限制性片段长度多态性分析将大多数临床血清型A和环境血清型B分离株分别归为分子类型VNI（98.1%）和VGII（100%）。99.3%的血清型A分离株为交配型α，而96.6%的血清型B分离株为交配型a。临床和环境分离株之间的相似特征表明，患者可能从环境中获得了感染。所提供的数据是哥伦比亚对正在进行的C. neoformans物种复合体。
The aim of this study was to investigate the epidemiological relationships of clinical and environmental isolates of the Cryptococcus neoformans species complex in Colombia. The current study reflects data from 1987 to 2004. In Colombia serotypes A and B are most frequently recovered from patients and the environment. Of the 178 clinical isolates studied, 91.1% were of serotype A, 8.4% serotype B and 0.5% serotype C. Of the 247 environmental isolates, 44.2% were of serotype A, 42.6% serotype B and 13.2% serotype C. No serotype D isolates were isolated. Serotype AD has not been recovered in Colombia. PCR fingerprinting with the primers M13, (GACA)(4) and (GTG)(5) and URA5 gene restriction fragment length polymorphism analysis grouped the majority of clinical serotype A and environmental serotype B isolates into the molecular types VNI (98.1%) and VGII (100%), respectively. Mating type alpha was determined in 99.3% of serotype A isolates, but 96.6% of serotype B isolates were of mating type a. Similar profiles between clinical and environmental isolates suggest that the patients may have acquired the infection from the environment. The data presented form part of the Colombian contribution to the ongoing global survey of the C. neoformans species complex.