WRKY transcription factors WRKY12 and WRKY13 interact with SPL10 to modulate age-mediated flowering
WRKY transcription factors WRKY12 and WRKY13 interact with SPL10 to modulate age-mediated flowering
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WRKY转录因子WRKY12和WRKY13与SPL10相互作用调节年龄介导的开花
DOI：
10.1111/jipb.12946
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发表时间：
2020-06-19
影响因子：
11.4
通讯作者：
Yu, Diqiu
中科院分区：
文献类型：
--
作者：
Ma, Zhenbing;Li, Wei;Yu, Diqiu
WRKY12和WRKY13是两个WRKY转录因子，在短日（SD）条件下的开花时间调控中起重要作用。wrky12和wrky13的暂时调控表达表明它们可能参与了年龄介导的开花途径。然而，人们对它们在这一途径中的作用知之甚少。在这里，我们发现wrky12和wrky13的转录是由SQUAMOSA启动子结合样10 （SPL10）直接调控的，SPL10是年龄通路下游的转录因子。结合和激活分析显示SPL10作为wrky12的正调控因子和wrky13的负调控因子。进一步的机制研究表明，WRKY12和WRKY13与SPL10物理相互作用，两者都结合到mir172b的启动子上。因此，WRKY12-SPL10和WRKY13-SPL10的相互作用分别促进和抑制SPL10的转录功能来调节emir172的表达。总之，我们的研究结果表明，WRKY12和WRKY13通过与SPLs物理相互作用并共同调节靶基因mir172b，参与SD条件下年龄介导的开花控制。
WRKY12 and WRKY13 are two WRKY transcription factors that play important roles in the control of flowering time under short-day (SD) conditions. The temporally regulated expression ofWRKY12andWRKY13indicates that they may be involved in the age-mediated flowering pathway. However, their roles in this pathway are poorly understood. Here, we show that the transcription ofWRKY12andWRKY13is directly regulated by SQUAMOSA PROMOTER BINDING-LIKE 10 (SPL10), a transcription factor downstream of the age pathway. Binding and activation analyses revealed that SPL10 functions as a positive regulator ofWRKY12and a negative regulator ofWRKY13. Further mechanistic investigation revealed that WRKY12 and WRKY13 physically interact with SPL10 and that both of them bind to the promoter ofmiR172b. Thus, the WRKY12-SPL10 and WRKY13-SPL10 interactions facilitate and inhibit SPL10 transcriptional function, respectively, to regulatemiR172bexpression. Together, our results show that WRKY12 and WRKY13 participate in the control of age-mediated flowering under SD conditions though physically interacting with SPLs and co-regulating the target genemiR172b.