LATERAL MOVEMENTS OF MEMBRANE-GLYCOPROTEINS RESTRICTED BY DYNAMIC CYTOPLASMIC BARRIERS
LATERAL MOVEMENTS OF MEMBRANE-GLYCOPROTEINS RESTRICTED BY DYNAMIC CYTOPLASMIC BARRIERS
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DOI：
10.1126/science.1835798
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发表时间：
1991-11-29
期刊：
影响因子：
56.9
通讯作者：
SHEETZ, MP
中科院分区：
文献类型：
--
作者：
EDIDIN, M;KUO, SC;SHEETZ, MP
细胞膜通常是片状的，由侧域组成。这些域可以由膜双层内或膜双层任一侧上的屏障形成。用抗体包被的金颗粒标记跨膜-（H-2D（B））或糖基磷脂酰肌醇（GPI）锚定（Qa 2）的主要组织相容性复合体（MHC）1类分子，并用激光光镊使其穿过细胞表面，直到它们遇到屏障，即无屏障路径长度（BFP）。在室温下，Qa 2和H-2D（B）的BFP分别为1.7 +/- 0.2和0.6 +/- 0.1（微米+/- SEM）。尽管两种MHC分子的BFP在34 ℃时比在23 ℃时高5倍，但在34 ℃时仍存在屏障。这表明，横向运动的障碍主要是在细胞质的一半膜和动态。
Cell membranes often are patchy, composed of lateral domains. These domains may be formed by barriers within or on either side of the membrane bilayer. Major histocompatibility complex (MHC) class 1 molecules that were either transmembrane- (H-2D(b)) or glycosylphosphatidylinositol (GPI)-anchored (Qa2) were labeled with antibody-coated gold particles and moved across the cell surface with a laser optical tweezers until they encountered a barrier, the barrier-free path length (BFP). At room temperature, the BFPs of Qa2 and H-2D(b) were 1.7 +/- 0.2 and 0.6 +/- 0.1 (micrometers +/- SEM), respectively. Barriers persisted at 34-degrees-C, although the BFP for both MHC molecules was fivefold greater at 34-degrees-C than at 23-degrees-C. This indicates that barriers to lateral movement are primarily on the cytoplasmic half of the membrane and are dynamic.