A Comparative Study on the Population Fitness of Three Strains of Nilaparvata lugens (Hemiptera: Delphacidae) Differ in Eye Color-Related Genes
A Comparative Study on the Population Fitness of Three Strains of Nilaparvata lugens (Hemiptera: Delphacidae) Differ in Eye Color-Related Genes
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DOI：
10.1093/jee/tov154
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发表时间：
2015-08
期刊：
影响因子：
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通讯作者：
Shu-Hua Liu;Baojun Yang;Ju Luo;Jian Tang;Jin‐cai Wu
中科院分区：
文献类型：
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作者：
Shu-Hua Liu;Baojun Yang;Ju Luo;Jian Tang;Jin‐cai Wu
褐飞虱是亚洲水稻上的一种毁灭性害虫。褐飞虱红眼突变体群体作为一种可见的遗传标记，是一种很有价值的材料。本研究通过连续8代的红眼母本与褐眼父本交配，建立了近等基因系NIL-BB和NIL-rr。生物学试验表明，NIL-BB的适合度代价较大，而NIL-rr的存活率和育性参数与实验室褐飞虱正常品系BB相当。NIL-BB个体产卵量和卵孵化率显著偏低是其适应度成本大的主要原因。BB、NIL-rr和NIL-BB的种群趋势指数分别为52.18、43.80和4.19。实时荧光定量PCR研究表明，NIL-BB的育性较差不是由卵子发生相关基因的差异表达介导的。与NIL-BB相比，NIL-rr的适应性更强，这可能是由于眼突变基因或与其紧密连锁的基因具有更强的生殖补偿能力所致。NIL-rr与BB的适合度相当，表明NIL-rr可用于田间研究。繁殖力和存活力显著下降的NIL-BB品系可作为研究繁殖力相关信号通路的模型。
ABSTRACT The brown planthopper, Nilaparvata lugens (Stål), is a destructive insect pest on rice throughout Asia. As a visible genetic marker, red eye mutant colony of brown planthopper is a valuable material. Here, we established the near-isogenic lines, NIL-BB and NIL-rr, through mating red eye females to brown eye brothers for eight successive generations. Biological experiments showed that NIL-BB had big fitness cost; however, NIL-rr had comparable survival and fertility parameters with BB, a normal laboratory brown planthopper strain. Significantly lower number eggs per female and egg hatchability were the key factors resulting in big fitness cost of NIL-BB. The population trend indexes of BB, NIL-rr, and NIL-BB were 52.18, 43.80, and 4.19, respectively. Real-time PCR study suggested that the poorer fertility of NIL-BB was not mediated by the differential expression of genes relating to oogenesis. The stronger fitness of NIL-rr compared with NIL-BB may be caused by the eye mutant gene or its closely linked genes having stronger compensation ability for reproduction. The comparable fitness of NIL-rr with BB indicated that NIL-rr may be used in field research. The NIL-BB strain with significantly declined fecundity and survival ability can be used as study model for the signal pathways relating to fecundity.