Resveratrol inhibition of varicella-zoster virus replication in vitro
Resveratrol inhibition of varicella-zoster virus replication in vitro
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DOI：
10.1016/j.antiviral.2006.07.004
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发表时间：
2006-12-01
影响因子：
7.6
通讯作者：
Booth, Tristan
中科院分区：
文献类型：
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作者：
Docherty, John J.;Sweet, Thomas J.;Booth, Tristan
白藜芦醇对水痘-带状疱疹病毒(VZV)复制的抑制作用呈剂量依赖性和可逆性。在白藜芦醇存在的情况下，病毒产量的下降不是由于VZV的直接灭活或抑制病毒与MRC-5细胞的附着造成的。如果在感染的前30小时内加入，该药物有效地限制了VZV的复制。Western印迹分析和实时RT-PCR研究表明，与对照组相比，IE62的蛋白质和mRNA水平降低。IE62是一种重要的即刻早期病毒蛋白。这些结果表明，白藜芦醇对VZV复制有负面影响，白藜芦醇对IE62合成有负面影响。(C)2006爱思唯尔B.V.保留所有权利。
Resveratrol was found to inhibit varicella-zoster virus (VZV) replication in a dose-dependent and reversible manner. This decrease in virus production in the presence of resveratrol was not caused by direct inactivation of VZV or inhibition of virus attachment to MRC-5 cells. The drug effectively limited VZV replication if added during the first 30 h of infection. Western blot analysis and real-time RT-PCR studies demonstrated that protein and mRNA levels of IE62, an essential immediate early viral protein, were reduced when compared to controls. These results;demonstrate that VZV replication is adversely affected by resveratrol which is negatively impacting IE62 synthesis. (c) 2006 Elsevier B.V All rights reserved.