A human rel proto-oncogene cDNA containing an Alu fragment as a potential coding exon.
A human rel proto-oncogene cDNA containing an Alu fragment as a potential coding exon.
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发表时间：
1989-07
期刊：
影响因子：
8
通讯作者：
E. Brownell;N. Mittereder;N. Rice
中科院分区：
文献类型：
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作者：
E. Brownell;N. Mittereder;N. Rice
从源自 Daudi 人类细胞系的文库中分离出两个含有 rel 的 cDNA 克隆，已知该细胞系表达 c-rel mRNA。克隆 #1 似乎包含完整的 c-rel 编码序列，其与 v-rel 的不同之处在于具有 3 个额外的 N 端残基和 111 个额外的 C 端残基。此外，克隆 #1 具有在 v-rel 或火鸡 c-rel 中未发现的内部 32 个氨基酸外显子。克隆 #2 在其 5' 末端被截断并且不包含这个新的外显子。对人类 c-rel 基因组克隆的分析表明，新的外显子是反向 Alu 重复序列的一部分。反向共有 Alu 序列中潜在剪接位点和开放阅读框的出现表明，Alu 片段作为潜在编码外显子的掺入可能是人类 mRNA 中相对常见的事件。这些信息是否可以被翻译尚不清楚：针对 c-rel 蛋白预测 C 末端的肽产生的抗血清沉淀了 p82hc-rel，但针对位于 Alu 外显子的肽产生的抗血清却没有。
Two rel-containing cDNA clones were isolated from a library derived from the Daudi human cell line, which is known to express c-rel mRNA. Clone #1 appeared to contain the entire c-rel coding sequence, which differs from v-rel in having three additional N-terminal residues and 111 additional C-terminal residues. In addition, Clone #1 had an internal 32 amino acid exon not found in v-rel or in turkey c-rel. Clone #2 was truncated at its 5' end and did not contain this new exon. Analysis of a genomic clone of human c-rel revealed that the new exon was a portion of an inverted Alu repeat. The occurrence of potential splice sites and of open reading frames in the inverted consensus Alu sequence suggests that the incorporation of Alu fragments as potential coding exons could be a relatively common event in human mRNAs. Whether such messages can be translated is unknown: antiserum raised against a peptide at the predicted C-terminus of the c-rel protein precipitated p82hc-rel, but antiserum raised against a peptide located in the Alu exon did not.