A simple system for staining protein and nucleic acid electrophoresis gels
A simple system for staining protein and nucleic acid electrophoresis gels
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DOI：
10.1002/elps.200600325
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发表时间：
2007-03
期刊：
影响因子：
2.9
通讯作者：
Dorian M. Raymer;Douglas E. Smith
中科院分区：
文献类型：
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作者：
Dorian M. Raymer;Douglas E. Smith
分子生物学的研究人员花费大量的时间倾向于电泳凝胶的染色和脱色。在这里，我们描述了一个简单的系统，成本100美元，并采取101小时的组装，自动化标准的核酸和蛋白质凝胶染色协议。染色在托盘中进行，或者在自动检测电压降后在电泳室本身中使用DNA凝胶进行。由微控制器芯片控制的微型泵以编程的时间间隔交换必要的溶液。我们证明了高效和高度重复性的溴化乙锭和亚甲蓝染色的DNA在琼脂糖凝胶和考马斯亮蓝和银染色的蛋白质在聚丙烯酰胺凝胶。
Researchers in molecular biology spend a significant amount of time tending to the staining and destaining of electrophoresis gels. Here we describe a simple system, costing ∼$100 and taking ∼1 h to assemble, that automates standard nucleic acid and protein gel staining protocols. Staining is done in a tray or, with DNA gels, in the electrophoresis chamber itself following automatic detection of the voltage drop. Miniature pumps controlled by a microcontroller chip exchange the necessary solutions at programmed time intervals. We demonstrate efficient and highly reproducible ethidium bromide and methylene blue staining of DNA in agarose gels and Coomassie blue and silver staining of proteins in polyacrylamide gels.