CALCIUM-CALMODULIN MODULATION OF THE OLFACTORY CYCLIC NUCLEOTIDE-GATED CATION CHANNEL
CALCIUM-CALMODULIN MODULATION OF THE OLFACTORY CYCLIC NUCLEOTIDE-GATED CATION CHANNEL
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DOI：
10.1126/science.266.5189.1348
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发表时间：
1994-11-25
期刊：
影响因子：
56.9
通讯作者：
YAU, KW
中科院分区：
文献类型：
--
作者：
LIU, MY;CHEN, TY;YAU, KW
虽然已有几种离子通道被报道直接受钙-钙调蛋白的调节，但它们尚未被确凿地证明与钙调蛋白结合，其调节机制也不清楚。对受钙-钙调蛋白调控的嗅觉环核苷酸激活的阳离子通道的研究表明，钙-钙调蛋白直接与通道氨基末端的特定结构域结合。这种结合降低了通道对环核苷酸的有效亲和力，显然是通过作用于与配体结合紧密耦合的通道门控来实现的。这些数据揭示了一种控制机制，类似于钙调素调节酶的机制。这些结果还表明，嗅觉通道的氨基末端部分是一个门控元件，这可能对具有相似整体结构的离子通道的运行具有普遍意义。
Although several ion channels have been reported to be directly modulated by calcium-calmodulin, they have not been conclusively shown to bind calmodulin, nor are the modulatory mechanisms understood. Study of the olfactory cyclic nucleotide-activated cation channel, which is modulated by calcium-calmodulin, indicates that calcium-calmodulin directly binds to a specific domain on the amino terminus of the channel. This binding reduces the effective affinity of the channel for cyclic nucleotides, apparently by acting on channel gating, which is tightly coupled to ligand binding. The data reveal a control mechanism that resembles those underlying the regulation of enzymes by calmodulin. The results also point to the amino-terminal part of the olfactory channel as an element for gating, which may have general significance in the operation of ion channels with similar overall structures.