Establishment and maintenance of heritable chromatin structure during early Drosophila embryogenesis.
Establishment and maintenance of heritable chromatin structure during early Drosophila embryogenesis.
复制标题
DOI：
10.7554/elife.20148
复制
发表时间：
2016-11-23
期刊：
影响因子：
7.7
通讯作者：
Wieschaus EF
中科院分区：
文献类型：
--
作者：
Blythe SA;Wieschaus EF
在胚胎发生期间，初始染色质状态在快速增殖活性期间建立。我们已经测量了3分钟的时间分辨率如何遗传模式的染色质结构最初建立和维持在囊胚中期过渡（MBT）。我们发现，区域的可访问性建立顺序，增强子在启动子和绝缘子之前打开。这些开放状态稳定地维持在高度浓缩的有丝分裂染色质中，以确保在细胞分裂中忠实地继承先前的可接近性状态。建立的时间进程由控制MBT发作的生物计时器控制。一般来说，启动子可及性的获得是由测量核质（N：C）比的生物计时器控制的，而增强子可及性的定时是独立于N：C比调节的。这些不同的时间类各自与两个转录因子的结合位点相关，GAGA因子和塞尔达，以前参与控制ZGA的染色质可及性。DOI：http://dx.doi.org/10.7554/eLife.20148.001网站
During embryogenesis, the initial chromatin state is established during a period of rapid proliferative activity. We have measured with 3-min time resolution how heritable patterns of chromatin structure are initially established and maintained during the midblastula transition (MBT). We find that regions of accessibility are established sequentially, where enhancers are opened in advance of promoters and insulators. These open states are stably maintained in highly condensed mitotic chromatin to ensure faithful inheritance of prior accessibility status across cell divisions. The temporal progression of establishment is controlled by the biological timers that control the onset of the MBT. In general, acquisition of promoter accessibility is controlled by the biological timer that measures the nucleo-cytoplasmic (N:C) ratio, whereas timing of enhancer accessibility is regulated independently of the N:C ratio. These different timing classes each associate with binding sites for two transcription factors, GAGA-factor and Zelda, previously implicated in controlling chromatin accessibility at ZGA. DOI: http://dx.doi.org/10.7554/eLife.20148.001