Synchronization of glycolytic oscillations in a yeast cell population
Synchronization of glycolytic oscillations in a yeast cell population
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DOI：
10.1039/b103238k
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发表时间：
2001-01-01
影响因子：
3.4
通讯作者：
Westerhoff, H
中科院分区：
文献类型：
--
作者：
Dano, S;Hynne, F;Westerhoff, H
近半个世纪以来，酵母细胞悬浮液中活性相同步的机制一直是个谜。问题的困难源于这样一个事实，即同步现象涉及糖酵解和发酵的整个代谢网络，因此不能在单一酶或单一化学物种的水平上解决。在本文中，我们展示了如何将CSTR(连续流动搅拌釜式反应器)中的这一系统定量地建模为一群Stuart-Landau振荡器通过细胞外介质中的代谢物交换而相互作用，从而在不牺牲生化真实性的情况下降低问题的复杂性。该模型的参数可以从具有超临界Hopf分叉的酵母细胞动力学的任何全尺度模型系统展开而得到。一些参数值也可以直接从微扰实验分析中获得。在平均场极限中，研究具有频率分布的种群的方程被用来模拟细胞间固有变化的影响。
The mechanism of active phase synchronization in a suspension of oscillatory yeast cells has remained a puzzle for almost half a century. The difficulty of the problem stems from the fact that the synchronization phenomenon involves the entire metabolic network of glycolysis and fermentation, and consequently it cannot be addressed at the level of a single enzyme or a single chemical species. In this paper it is shown how this system in a CSTR (continuous flow stirred tank reactor) can be modelled quantitatively as a population of Stuart-Landau oscillators interacting by exchange of metabolites through the extracellular medium, thus reducing the complexity of the problem without sacrificing the biochemical realism. The parameters of the model can be derived by a systematic expansion from any full-scale model of the yeast cell kinetics with a supercritical Hopf bifurcation. Some parameter values can also be obtained directly from analysis of perturbation experiments. In the mean-field limit, equations for the study of populations having a distribution of frequencies are used to simulate the effect of the inherent variations between cells.