Estrogen receptor in adult male rat liver.
Estrogen receptor in adult male rat liver.
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成年雄性大鼠肝脏中的雌激素受体。
DOI：
10.1210/endo-103-5-1629
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发表时间：
1978
期刊：
影响因子：
4.8
通讯作者：
A. Eisenfeld
中科院分区：
文献类型：
--
作者：
R. Aten;R. Dickson;A. Eisenfeld
30%硫酸铵沉淀以前已被用于部分纯化雌性大鼠肝细胞质中的雌激素受体。这个程序现在已被用来fractionate雌激素结合位点的成年雄性大鼠肝胞质溶胶。30%硫酸铵沉淀部分纯化了一组雌二醇结合位点，其性质与雄性肝胞质溶胶中存在的大量位点完全不同。部分纯化的男性网站似乎具有相同的属性作为部分纯化的女性雌二醇结合位点。它们似乎是雌激素特异性蛋白质，具有高雌二醇亲和力（Kd = 1 × 10（-10）M）和低雌激素容量（2.3 fmol/mg肝脏）。青春期前雄性大鼠肝胞质溶胶的雌二醇结合位点也被30%硫酸铵沉淀分离。青春期前雄性大鼠肝胞液中硫酸铵沉淀物的雌二醇结合位点比成年雄性或雌性大鼠少。成年雄性大鼠和成年雌性大鼠的肝脏似乎都含有雌激素受体。
A 30% ammonium sulfate precipitation has previously been utilized to partially purify the estrogen receptor(s) of female rat liver cytosol. This procedure has now been used to fractionate the estradiol-binding sites of adult male rat liver cytosol. The 30% ammonium sulfate precipitation partially purifies a group of estradiol-binding sites which have properties quite distinct from the large number of sites present in male liver cytosol. The partially purified male sites seem to have the same properties as the partially purified female estradiol-binding sites. They seem to be proteins that are estrogen specific and have a high estradiol affinity (Kd = 1 X 10(-10) M) and a low estrogen capacity (2.3 fmol/mg liver). The estradiol-binding sites of prepubescent male rat liver cytosol have also been fractionated by 30% ammonium sulfate precipitation. The redissolved ammonium sulfate precipitates from prepubescent male rat liver cytosol contain fewer estradiol-binding sites then those from adult male or female rats. It seems that adult male as well as adult female rat liver contains estrogen receptors.