Transforming growth factor-β1 regulates Kir2.3 inward rectifier K+ channels via phospholipase C and protein kinase C-δ in reactive astrocytes from adult rat brain
Transforming growth factor-β1 regulates Kir2.3 inward rectifier K+ channels via phospholipase C and protein kinase C-δ in reactive astrocytes from adult rat brain
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DOI：
10.1074/jbc.m107984200
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发表时间：
2002-01-18
影响因子：
4.8
通讯作者：
Simard, JM
中科院分区：
文献类型：
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作者：
Perillan, PR;Chen, MK;Simard, JM
多功能细胞因子转化生长因子β (1) （tgf - β(1)）对星形胶质细胞具有复杂的影响，其早期信号事件的特征不如转录机制那么明确。我们检测了tgf - β(1)对大鼠原代培养的反应性星形胶质细胞14-pS Kir2.3内向整流K+通道的影响。免疫荧光研究显示，细胞共表达tgf - β(1)受体I和2、Kir2.3和胶质纤维酸性蛋白（GFAP）。膜片钳研究显示，tgf - β (1) （0.1-100 ng/ml）可引起
The multifunctional cytokine, transforming growth factor beta(1) (TGF-beta(1)), exerts complex effects on astrocytes with early signaling events being less well characterized than transcriptional mechanisms. We examined the effect of TGF-beta(1) on the 14-pS Kir2.3 inward rectifier K+ channel in rat primary cultured reactive astrocytes. Immunofluoreseence study showed that cells co-expressed TGF-beta(1) receptors I and 2, Kir2.3, and glial fibrillary acidic protein (GFAP). Patch clamp study showed that TGF-beta(1) (0.1-100 ng/ml) caused a rapid (