Identification of the genes involved in odorant reception and detection in the palm weevil Rhynchophorus ferrugineus, an important quarantine pest, by antennal transcriptome analysis.
Identification of the genes involved in odorant reception and detection in the palm weevil Rhynchophorus ferrugineus, an important quarantine pest, by antennal transcriptome analysis.
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DOI：
10.1186/s12864-016-2362-6
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发表时间：
2016-01-22
期刊：
影响因子：
4.4
通讯作者：
Pain A
中科院分区：
文献类型：
--
作者：
Antony B;Soffan A;Jakše J;Abdelazim MM;Aldosari SA;Aldawood AS;Pain A
红棕象甲Rhynchophorus ferrugineus（奥利弗Oliver）是世界上危害最大的入侵昆虫之一。这种象鼻虫是高度专业化的，在恶劣的沙漠气候中茁壮成长，由于它对世界各地的棕榈树的影响，它造成了重大的经济损失。RPW通过植物挥发性线索定位棕榈树，并使用聚合信息素来协调大规模攻击。在这里，我们报告的RPW触角转录组的高通量测序，并提出了高度表达的化学感受基因家族的描述。RPW触角转录组的深度测序和组装产生了35，667个平均长度为857 bp的转录物，并鉴定了大量高度表达的气味结合蛋白（OBP），化学感受蛋白（CSP），气味受体/共受体（OR/Orcos），感觉神经元膜蛋白（SNMP），味觉受体（GR）和离子型受体（IR）的转录物。在R中总共注释了38个OBP、12个CSP、76个OR、1个Orco、6个SNMP、15个GR和10个IR。锈色菌触角转录组与小蠹的比较转录组分析显示，25%的爆炸命中是小蠹独有的。ferrugineus，表明R.铁锈色的。我们将RPW OR分为7个鞘翅目OR亚科，并预测了两个新的OR亚科。的OR蛋白序列进行了比较与面粉甲虫，天牛甲虫和树皮甲虫，我们确定了鞘翅目特异性的，高度保守的OR以及独特的OR是puperies参与RPW聚集信息素检测。我们确定了26个负C OBP和8个正C OBP，并将R分组。ferrugineus OBP根据亲缘关系分为不同的OBP亚科，表明在R.铁锈色的。我们还确定了一个不同的CSP蛋白家族，以及一个鞘翅目特异性CSP谱系，从双翅目和鳞翅目分化。我们鉴定了几个极端分歧的IR直向同源物以及高度保守的昆虫IR共受体直向同源物转录本。铁锈色的。值得注意的是，在R. ferrugineus，我们发现了鞘翅目家庭内的GR的巨大多样性。对于SNMP-1和SNMP-2同源转录物，发现一个SNMP-1同源物在R.锈色菌触角转录组我们的研究提出了第一个全面的目录嗅觉基因家族参与信息素和一般气味检测在R。ferrugineus，这是潜在的新的目标害虫控制策略。本文的在线版本（doi：10.1186/s12864 - 016 - 2362 - 6）包含补充材料，可供授权用户使用。
The Red Palm Weevil (RPW) Rhynchophorus ferrugineus (Oliver) is one of the most damaging invasive insect species in the world. This weevil is highly specialized to thrive in adverse desert climates, and it causes major economic losses due to its effects on palm trees around the world. RPWs locate palm trees by means of plant volatile cues and use an aggregation pheromone to coordinate a mass-attack. Here we report on the high throughput sequencing of the RPW antennal transcriptome and present a description of the highly expressed chemosensory gene families. Deep sequencing and assembly of the RPW antennal transcriptome yielded 35,667 transcripts with an average length of 857 bp and identified a large number of highly expressed transcripts of odorant binding proteins (OBPs), chemosensory proteins (CSPs), odorant receptors/co-receptors (ORs/Orcos), sensory neuron membrane proteins (SNMPs), gustatory receptors (GRs) and ionotropic receptors (IRs). In total, 38 OBPs, 12 CSPs, 76 ORs, 1 Orco, 6 SNMPs, 15 GRs and 10 IRs were annotated in the R. ferrugineus antennal transcriptome. A comparative transcriptome analysis with the bark beetle showed that 25 % of the blast hits were unique to R. ferrugineus, indicating a higher, more complete transcript coverage for R. ferrugineus. We categorized the RPW ORs into seven subfamilies of coleopteran ORs and predicted two new subfamilies of ORs. The OR protein sequences were compared with those of the flour beetle, the cerambycid beetle and the bark beetle, and we identified coleopteran-specific, highly conserved ORs as well as unique ORs that are putatively involved in RPW aggregation pheromone detection. We identified 26 Minus-C OBPs and 8 Plus-C OBPs and grouped R. ferrugineus OBPs into different OBP-subfamilies according to phylogeny, which indicated significant species-specific expansion and divergence in R. ferrugineus. We also identified a diverse family of CSP proteins, as well as a coleopteran-specific CSP lineage that diverged from Diptera and Lepidoptera. We identified several extremely diverged IR orthologues as well as highly conserved insect IR co-receptor orthologous transcripts in R. ferrugineus. Notably, GR orthologous transcripts for CO2-sensing and sweet tastants were identified in R. ferrugineus, and we found a great diversity of GRs within the coleopteran family. With respect to SNMP-1 and SNMP-2 orthologous transcripts, one SNMP-1 orthologue was found to be strikingly highly expressed in the R. ferrugineus antennal transcriptome. Our study presents the first comprehensive catalogue of olfactory gene families involved in pheromone and general odorant detection in R. ferrugineus, which are potential novel targets for pest control strategies. The online version of this article (doi:10.1186/s12864-016-2362-6) contains supplementary material, which is available to authorized users.