Integrating silicon/zinc dual elements with PLGA microspheres in calcium phosphate cement scaffolds synergistically enhances bone regeneration
Integrating silicon/zinc dual elements with PLGA microspheres in calcium phosphate cement scaffolds synergistically enhances bone regeneration
复制标题
将硅/锌双元素与 PLGA 微球集成在磷酸钙水泥支架中可协同增强骨再生
DOI：
10.1039/c9tb02901j
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发表时间：
2020-04-21
影响因子：
7
通讯作者：
Deng, Xuliang
中科院分区：
文献类型：
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作者：
Liang, Weiwei;Gao, Min;Deng, Xuliang
将多种促成骨因子整合到骨移植替代物中是提高骨修复效果的一种切实有效的方法。在这里，硅锌双元素和PLGA微球被纳入磷酸钙骨水泥（CPC）支架（PLGA/CPC-Si/Zn）作为一种新的策略，以协同促进骨再生。PLGA微球和Si/Zn双元素的加入改善了CPC的凝固时间、可注射性和抗压强度。PLGA/CPC-Si/Zn支架显示出Si和Zn离子的受控顺序释放。在体外，RAW 264.7细胞显示出M2表型，并响应于PLGA/CPC-Si/Zn而具有高水平的抗炎细胞因子。RAW 264.7细胞条件培养液在PLGA/CPC-Si/Zn支架上培养可显著促进大鼠BMSCs的成骨分化。在大鼠股骨缺损模型中，植入PLGA/CPC-Si/Zn支架4周后导致明显的新骨形成，12周后明显的骨长入PLGA微球中，24周时PLGA微球降解后几乎完全被成熟的新骨填充。这些发现表明PLGA/CPC-Si/Zn支架通过协同改善免疫微环境和生物降解性来促进骨生成。因此，将多种微量元素与可降解组分一起整合在骨移植生物材料中可以是促进骨再生的有效策略。
Integrating multiple pro-osteogenic factors into bone graft substitutes is a practical and effective approach to improve bone repair efficacy. Here, Si-Zn dual elements and PLGA microspheres were incorporated into calcium phosphate cement (CPC) scaffolds (PLGA/CPC-Si/Zn) as a novel strategy to synergistically enhance bone regeneration. The incorporation of PLGA microspheres and Si/Zn dual elements within CPC scaffolds improved the setting time, injectability and compressive strength. The PLGA/CPC-Si/Zn scaffolds displayed controlled sequential release of Si and Zn ions. In vitro, RAW 264.7 cells displayed the M2 phenotype with a high level of anti-inflammatory cytokines in response to PLGA/CPC-Si/Zn. The conditioned medium of RAW 264.7 cells cultured on the PLGA/CPC-Si/Zn scaffolds significantly enhanced the osteogenic differentiation of rat BMSCs. In a rat femur defect model, the implanted PLGA/CPC-Si/Zn scaffolds led to obvious new bone formation after 4 weeks, apparent bone ingrowth into the PLGA microspheres after 12 weeks, and was almost completely filled with mature new bone upon degradation of the PLGA microspheres at 24 weeks. These findings demonstrate that the PLGA/CPC-Si/Zn scaffolds promote osteogenesis by synergistically improving the immune microenvironment and biodegradability. Hence, integrating multiple trace elements together with degradable components within bone graft biomaterials can be an effective strategy for promoting bone regeneration.