INCREASED LEVELS OF INFLAMMATORY CYTOKINES IN HUMAN SKIN LYMPH DERIVED FROM SODIUM LAURYL SULFATE-INDUCED CONTACT-DERMATITIS
INCREASED LEVELS OF INFLAMMATORY CYTOKINES IN HUMAN SKIN LYMPH DERIVED FROM SODIUM LAURYL SULFATE-INDUCED CONTACT-DERMATITIS
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DOI：
10.1111/j.1365-2133.1992.tb00123.x
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发表时间：
1992-09-01
影响因子：
10.3
通讯作者：
BRAATHEN, LR
中科院分区：
文献类型：
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作者：
HUNZIKER, T;BRAND, CU;BRAATHEN, LR
在6名健康志愿者的小腿上插管引流足部上部和内侧皮肤的浅表外周淋巴管。2天后，通过向淋巴管引流的皮肤区域施用10%十二烷基硫酸钠诱导刺激性接触性皮炎。三天后，用丙酸氯倍他索治疗自发消退的皮肤反应。每天两次收集淋巴液，持续7天，并通过ELISA技术测定各种细胞因子（IL-1-α、IL-1-β、IL-2和可溶性IL-2受体、IL-6、IL-8、TNF-α、GM-CSF）的水平。在大多数志愿者中，除IL-1-α和IL-8外，在几个淋巴样品中检测到所有检查的细胞因子。与接触性皮炎的临床症状平行，IL-6和TNF-α的水平增加8-10倍，而IL-1-β、IL-2、IL-2受体和GM-CSF的水平延迟增加2-3倍。这些结果表明，细胞因子，特别是IL-6和TNF-α，可能积极参与在接触性皮炎的皮肤和局部淋巴结的免疫反应。
A superficial peripheral lymph vessel draining the skin of the upper and medial part of the foot was cannulated on the lower leg of six healthy human volunteers. After 2 days an irritant contact dermatitis was induced by application of 10% sodium lauryl sulphate to the area of skin drained by the lymph vessel. Three days later the spontaneously regressing skin reaction was treated with clobetasol propionate. Lymph was collected twice daily for 7 days, and the levels of various cytokines (IL-1-alpha, IL-1-beta, IL-2 and soluble IL-2 receptors, IL-6, IL-8, TNF-alpha, GM-CSF) were determined by ELISA technique. In the majority of the volunteers all cytokines examined were detected in several lymph samples, with the exception of IL-1-alpha and IL-8. In parallel with the clinical symptoms of the contact dermatitis the levels of IL-6 and TNF-alpha increased 8-10-fold, whereas for IL-1-beta, IL-2, IL-2 receptors, and GM-CSF there was a delayed, 2-3-fold increase. These results suggest that cytokines, in particular IL-6 and TNF-alpha, may actively participate in the immunological reactions in the skin and in the regional lymph nodes during contact dermatitis.