Fibronectin deficiency and intestinal transvascular fluid balance during bacteremia.
Fibronectin deficiency and intestinal transvascular fluid balance during bacteremia.
复制标题
菌血症期间纤连蛋白缺乏和肠道经血管液体平衡。
DOI：
10.1152/ajpheart.1982.242.4.h557
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Saba,TM
中科院分区：
文献类型：
--
作者：
Dillon,BC;Saba,TM
网状内皮（RE）清除功能障碍，这可能是由调理素纤维连接蛋白缺乏引起的，已与脓毒症期间的器官衰竭。我们研究调理素纤维连接蛋白缺乏和RE封锁的作用，在调节肠经血管液体平衡的改变假单胞菌血症诱导使用一个孤立的，神经支配，和自动灌注犬小肠段。静脉输注明胶包被颗粒用于诱导纤维连接蛋白缺乏和RE阻断。淋巴流量和淋巴/血浆（L/P）蛋白浓度比稳定后，静脉内挑战与细菌或明胶涂层颗粒。相反，当菌血症与颗粒诱导的调理素纤维连接蛋白缺乏和RE阻断共存时，淋巴流量增加，L/P比值显著降低。淋巴流量的增加和L/P比值的下降与血管对白蛋白、IgG和IgM的正常渗透性有关。RE阻断菌血症期间肠液流量增加似乎是由于微血管静水压力增加，而不是血管通透性增加。这些发现强调了纤连蛋白和相关RE系统功能作为脓毒症期间液体过滤决定因素的潜在重要作用。
Reticuloendothelial (RE) clearance dysfunction, which can be induced by opsonic fibronectin deficiency, has been correlated with organ failure during sepsis. We investigate the role of opsonic fibronectin deficiency and RE blockade in modulating alterations in intestinal transvascular fluid balance induced by Pseudomonas bacteremia using an isolated, innervated, and autoperfused canine small intestinal segment. Intravenous infusion of gelatin-coated particles was used to induce fibronectin deficiency and RE blockade. Lymph flow and lymph/plasma (L/P) protein concentration ratios were stable following intravenous challenge with bacteria or gelatin-coated particles. In contrast, lymph flow increased and L/P ratio decreased significantly when bacteremia coexisted with particle-induced opsonic fibronectin deficiency and RE blockade. This elevation in lymph flow and decline in L/P ratio was associated with normal vascular permeability to albumin, IgG, and IgM. The increase in intestinal fluid flux during bacteremia with RE blockade appears to be due to an increase in microvascular hydrostatic pressure and not to an increase in vascular permeability. These findings emphasize a potentially important role for fibronectin and associated RE system function as determinants of fluid filtration during sepsis.