Dental Mesenchymal Stem Cells: Dental Pulp Stem Cells, Periodontal Ligament Stem Cells, Apical Papilla Stem Cells, and Primary Teeth Stem Cells-Isolation, Characterization, and Expansion for Tissue Engineering
Dental Mesenchymal Stem Cells: Dental Pulp Stem Cells, Periodontal Ligament Stem Cells, Apical Papilla Stem Cells, and Primary Teeth Stem Cells-Isolation, Characterization, and Expansion for Tissue Engineering
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DOI：
10.1007/978-1-4939-9012-2_7
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发表时间：
2019-01-01
期刊：
影响因子：
--
通讯作者：
Huang, George T. -J.
中科院分区：
文献类型：
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作者：
Al-Habib, Mey;Huang, George T. -J.
牙齿干细胞（DentalStemCells，DSCs）在生物医学领域有着广泛的应用前景，尤其是在牙齿组织再生方面。它们是间充质干细胞/基质细胞（MSC）的一种特殊类型的亚群，与其他类型的MSC存在细微差异。因此，它需要一个专门的专业知识来分离，培养，并在体外和体内表征这些细胞。本章的目的是分享我们研究这些细胞的经验。我们将详细描述实验室方案，概述细胞是如何分离，培养，扩增，并使用各种体外细胞和生化分析，以及体内研究模型，使用免疫功能低下的小鼠观察这些DSCs移植后的组织再生。
Dental stem cells (DSCs) have been shown to possess great potential for multiple biomedical applications, especially for dental tissue regeneration. They are a special type of subpopulation of mesenchymal stem/stromal cells (MSCs) and present subtle differences from other types of MSCs. Therefore, it requires a specialized expertise to isolate, culture, and characterize these cells in vitro and in vivo. The purpose of this chapter is to share our experience in studying these cells. We will describe in detail laboratory protocols outlining how the cells are isolated, cultured, expanded, and characterized using various in vitro cellular and biochemical analyses, as well as an in vivo study model using immunocompromised mice to observe tissue regeneration after transplantation of these DSCs.