Cleaved inflammatory lactoferrin peptides in parotid saliva of periodontitis patients
Cleaved inflammatory lactoferrin peptides in parotid saliva of periodontitis patients
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DOI：
10.1016/j.molimm.2006.09.003
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发表时间：
2007-03-01
影响因子：
3.6
通讯作者：
Sugawara, Shunji
中科院分区：
文献类型：
--
作者：
Komine, Ken-ichi;Kuroishi, Toshinobu;Sugawara, Shunji
乳铁蛋白 (Lf) 是铁结合抗菌蛋白转铁蛋白家族的成员，存在于大多数外分泌分泌物中，例如唾液，在粘膜防御中发挥重要作用。在本研究中，我们使用抗人Lf多克隆抗体，通过Con A二维免疫电泳、Con A亲和层析和Western blotting，鉴定了慢性牙周炎患者腮腺唾液中具有Con A低亲和力的小Lf肽。四种Con A低亲和力Lf肽的N端氨基酸测序证实它们是完整U的片段。牙周炎患者腮腺唾液中蛋白酶3（PR3）样活性的检出率升高，并且与临床症状的严重程度相关。在牙周炎患者的腮腺唾液中也检测到了PR3蛋白，并且PR3而不是人白细胞弹性蛋白酶和组织蛋白酶G降解了完整的Lf。 Con A 低亲和力唾液 Lf 肽对大肠杆菌没有抗菌活性，并且铁螯合能力降低。 Con A 低亲和力唾液 Lf 肽、PR3 处理的 Lf 制剂和四种合成多肽中的两种诱导人口腔上皮 HSC-2 细胞中白介素 IL-6、单核细胞趋化蛋白-1 和 IL-8 的产生以及 NF-κ B 的激活。此外，牙周炎患者腮腺唾液中 Lf 肽的浓度升高，且与临床症状的严重程度相关。这些结果表明，牙周炎患者腮腺唾液中的Lf被PR3样活性降解为小肽，具有诱导炎症介质的能力。 (c) 2006 Elsevier Ltd. 保留所有权利。
Lactoferrin (Lf) is a member of the transferrin family of iron-binding anti-bacterial proteins, present in most exocrine secretions, such as saliva, and plays an important role in mucosal defense. In this study, we identified small Lf peptides with Con A low-affinity in the parotid saliva of chronic periodontitis patients by Con A two-dimensional immunoelectrophoresis, Con A affinity chromatography and Western blotting using anti-human Lf polyclonal Ab. N-terminal amino acid sequencing of the four Con A low-affinity Lf peptides confirmed them to be fragments of intact U. The detection ratio of the proteinase 3 (PR3)-like activity was elevated in the parotid saliva of periodontitis patients and was associated with the severity of clinical symptoms. PR3 protein was also detected in the parotid saliva of periodontitis patients, and PR3, but not human leukocyte elastase and cathepsin G, degraded intact Lf. Con A low-affinity saliva Lf peptides showed no anti-bacterial activity against Escherichia coli, and had a reduced iron-chelating capacity. Con A low-affinity saliva Lf peptides, PR3-treated Lf preparation and two of four synthetic polypeptides induced the production of interleukin IL-6, monocyte chemoattractant protein-1 and IL-8, and the activation of NF-kappa B in human oral epithelial HSC-2 cells. Furthermore, concentrations of the Lf peptides in the parotid saliva of periodontitis patients were increased with a correlation to the severity of clinical symptoms. These results suggest that Lf in the parotid saliva of periodontitis patients was degraded into small peptides by the PR3-like activity with the capability to induce inflammatory mediators. (c) 2006 Elsevier Ltd. All rights reserved.