Tannin-rich pomegranate rind extracts reduce adhesion to and invasion of Caco-2 Cells by Listeria monocytogenes and decrease its expression of virulence genes.
Tannin-rich pomegranate rind extracts reduce adhesion to and invasion of Caco-2 Cells by Listeria monocytogenes and decrease its expression of virulence genes.
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DOI：
10.4315/0362-028x.jfp-14-174
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发表时间：
2015
影响因子：
2
通讯作者：
Yunfeng Xu;Guanghui Li;Baigang Zhang;Qian Wu;Xin Wang;X. Xia
中科院分区：
文献类型：
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作者：
Yunfeng Xu;Guanghui Li;Baigang Zhang;Qian Wu;Xin Wang;X. Xia
石榴皮富含单宁，具有显著的抗菌活性。本研究旨在评估石榴皮富含单宁的组分（TFPR）对单核细胞增生李斯特菌毒力基因表达以及病原体与人类上皮细胞相互作用的影响。监测生长曲线以确定TFPR对L.单核细胞增生采用3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四氮唑溴化物和荧光染色法检测TFPR的细胞毒性。研究了TFPR对L.使用Caco-2细胞研究单核细胞增多症对上皮细胞的粘附和侵袭。采用实时荧光定量PCR技术对3个毒力基因的mRNA水平进行了定量分析。单核细胞增多症结果表明，TFPR对L.单核细胞增多症在本研究中为5 mg/ml。当TFPR浓度为2.5mg/ml时，其对Caco-2细胞具有细胞毒性。亚抑菌浓度的TFPR以剂量依赖性方式显著降低乳酸杆菌对Caco-2细胞的粘附和侵袭。单核细胞增多症当L.当单核细胞增多症在2.5mg/ml TFPR存在下生长时，prfA、inlA和hly的转录水平分别降低17倍、34倍和28倍。
Pomegranate rind is rich in tannins that have remarkable antimicrobial activities. This study aimed to evaluate the effects of a tannin-rich fraction from pomegranate rind (TFPR) on Listeria monocytogenes virulence gene expression and on the pathogen's interaction with human epithelial cells. Growth curves were monitored to determine the effect of TFPR on L. monocytogenes growth. The 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide and fluorescence staining assays were used to examine the cytotoxicity of TFPR. The effects of TFPR on L. monocytogenes adhesion to and invasion of epithelial cells were investigated using Caco-2 cells. Real-time quantitative PCR analysis was conducted to quantify mRNA levels of three virulence genes in L. monocytogenes. Results showed that a MIC of TFPR against L. monocytogenes was 5 mg/ml in this study. TFPR exhibited cytotoxicity against Caco-2 cells when the concentration was 2.5 mg/ml. Subinhibitory concentrations of TFPR significantly reduced, in a dose-dependent manner, adhesion to and invasion of Caco-2 cells by L. monocytogenes. When L. monocytogenes was grown in the presence of 2.5 mg/ml TFPR, the transcriptional levels of prfA, inlA, and hly decreased by 17-, 34-, and 28-fold, respectively.