NUCLEOTIDE-SEQUENCE AND FUNCTIONAL-ANALYSIS OF THE META-CLEAVAGE PATHWAY INVOLVED IN BIPHENYL AND POLYCHLORINATED BIPHENYL DEGRADATION IN PSEUDOMONAS SP STRAIN KKS102
NUCLEOTIDE-SEQUENCE AND FUNCTIONAL-ANALYSIS OF THE META-CLEAVAGE PATHWAY INVOLVED IN BIPHENYL AND POLYCHLORINATED BIPHENYL DEGRADATION IN PSEUDOMONAS SP STRAIN KKS102
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DOI：
10.1128/jb.176.14.4269-4276.1994
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发表时间：
1994-07-01
影响因子：
3.2
通讯作者：
TAKAGI, M
中科院分区：
文献类型：
--
作者：
KIKUCHI, Y;YASUKOCHI, Y;TAKAGI, M
假单胞菌属菌株KKS102能够通过间位裂解途径降解联苯和多氯联苯。我们对bphA1A2A3BCD(开放阅读框架1[ORF1])A4的上游区域进行了测序，发现该区域有4个ORF。因为第一、第二和第三个开放阅读框推导的氨基酸序列与假单胞菌的间裂解酶同源。菌株CF600(V.Shingler，J.Powlowski和U.Marklund，J.Baciol，174：711-724,1992)，这些ORF分别被命名为bphE、bphG和bphF。第四个ORF(ORF4)与产碱假单胞菌KF707(K.Taira，J.Hirose，S.Hayashida和K.Furukawa，J.Biot)的ORF3同源。化学。267：4844-4853,1992)，其功能未知。以表达编码基因的大肠杆菌粗提物为材料，分析了BphE、BphG和BphF三种代谢裂解酶的功能。结果表明，bphE、bphG和bphF分别编码2-羟基戊酸-2，4-二烯酸水合酶、乙醛脱氢酶(酰化)和4-羟基-2-草戊酸缩醛酶。KKS102对联苯和多氯联苯的降解途径由12个基因编码，顺序为bphEGF(ORF4)A1A2A3BCD(ORF1)A4。ORF1和ORF4的功能尚不清楚。并对该基因簇的特点进行了讨论。
Pseudomonas sp. strain KKS102 is able to degrade biphenyl and polychlorinated biphenyls via the meta-cleavage pathway. We sequenced the upstream region of the bphA1A2A3BCD (open reading frame 1 [ORF1]) A4 and found four ORFs in this region. As the deduced amino acid sequences of the first, second, and third ORFs are homologous to the meta-cleavage enzymes from Pseudomonas sp. strain CF600 (V. Shingler, J. Powlowski, and U. Marklund, J. Bacteriol, 174:711-724, 1992), these ORFs have been named bphE, bphG, and bphF, respectively. The fourth ORF (ORF4) showed homology with ORF3 from Pseudomonas pseudoalcaligenes KF707 (K. Taira, J. Hirose, S. Hayashida, and K. Furukawa, J. Biot. Chem. 267:4844-4853, 1992), whose function is unknown. The functions of meta-cleavage enzymes (BphE, BphG, and BphF) were analyzed by using crude extracts of Escherichia coli which expressed the encoding genes. The results showed that bphE, bphG, and bphF encode 2-hydroxypenta-2,4-dienoate hydratase, acetaldehyde dehydrogenase (acylating), and 4-hydroxy-2- oxovalerate aldolase, respectively. The biphenyl and polychlorinated biphenyl degradation pathway of KKS102. is encoded by 12 genes in the order bphEGF (ORF4)A1A2A3BCD (ORF1)A4. The functions of ORF1 and ORF4 are unknown. The features of this bph gene cluster are discussed.