Maternally expressed PGK-Cre transgene as a tool for early and uniform activation of the Cre site-specific recombinase
Maternally expressed PGK-Cre transgene as a tool for early and uniform activation of the Cre site-specific recombinase
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DOI：
10.1023/a:1008868325009
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发表时间：
1998-03-01
影响因子：
3
通讯作者：
Lonai, P
中科院分区：
文献类型：
--
作者：
Lallemand, Y;Luria, V;Lonai, P
描述了具有Cre位点特异性重组酶的早期和均匀表达的转基因小鼠品系。在该菌株PGK-Cre（m）中，Cre由早期作用的PGK-1启动子驱动，但可能由于整合位点处的顺式效应，重组酶处于显性母体控制下。当Cre通过PGK-Cre（m）传递时，雌性与携带侧接loxP位点的报告基因转基因的雄性交配，即使不遗传PGK-Cre的后代也会删除靶基因。因此，在PGK-Cre（m）雌性Cre活性开始于卵子发生的二倍体阶段。在PGK-Cre（m）杂交中，在所有器官中观察到完全重组，包括睾丸和卵巢。我们制备了PGK-Cre（m）和白化病（c）基因座纯合的小鼠原种。该菌株将用于异位和显性负突变的早期和均匀诱导，用于从靶向突变中体内去除选择性元件，以及与在“敲入”和相关技术中操纵靶向基因座有关。
A transgenic mouse strain with early and uniform expression of the Cre site-specific recombinase is described. In this strain, PGK-Cre(m), Cre is driven by the early acting PGK-1 promoter, but, probably due to cis effects at the integration site, the recombinase is under dominant maternal control. When Cre is transmitted by PGK-Cre(m) females mated to males that carry a reporter transgene flanked by loxP sites, even offspring that do not inherit PGK-Cre delete the target gene. It follows that in the PGK-Cre(m) female Cre activity commences in the diploid phase of oogenesis. In PGK-Cre(m) crosses complete recombination was observed in all organs, including testis and ovary. We prepared a mouse stock that is homozygous for PGK-Cre(m) and at the albino (c) locus. This strain will be useful for the early and uniform induction of ectopic and dominant negative mutations, for the in vivo removal of selective elements from targeted mutations and in connection with the manipulation of targeted loci in `knock in' and related technologies.