Identification of differentially expressed genes like cofilin2 in growing collateral arteries
Identification of differentially expressed genes like cofilin2 in growing collateral arteries
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DOI：
10.1016/s0006-291x(02)02921-2
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发表时间：
2003-01-17
影响因子：
3.1
通讯作者：
Deindl, E
中科院分区：
文献类型：
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作者：
Boengler, K;Pipp, F;Deindl, E
兔股动脉闭塞可诱导原有侧支动脉的生长。目的：分析动脉形成过程中基因的差异表达。对股骨闭塞或假手术后24h的侧支动脉进行抑制减法杂交（SSH）。我们发现，在股动脉结扎24小时后，侧枝动脉中U6 snRNA结合蛋白Lsm5、细胞色素b、表达序列标签和肌动蛋白解聚因子cofilin2 mRNA上调。对于cofilin2，我们也检测到蛋白水平的增加，并且主要定位于侧枝平滑肌细胞。在cofilin2上调的同时，我们发现在生长的侧支动脉中α -平滑肌肌动蛋白mRNA下调。综上所述，我们的数据显示了参与动脉形成不同基本过程的基因表达水平的增强。(C) 2002 Elsevier Science （USA）。版权所有。
Arteriogenesis, the growth of pre-existing collateral arteries, can be induced in rabbits by occlusion of the femoral artery. In order to analyze the differential gene expression in arteriogenesis. cDNA of collateral arteries 24h after femoral occlusion or sham operation was subjected to suppression subtractive hybridization (SSH). We demonstrated an upregulation of the U6 snRNA binding protein Lsm5, cytochrome b, an expressed sequence tag, and the actin-depolymerizing factor cofilin2 mRNA in collateral arteries 24h after femoral ligation. For cofilin2, we also detected an increase in the protein level and a localization predominantly in smooth muscle cells of collaterals. Simultaneously with the upregulation of cofilin2 we found a downregulation of the alpha-smooth muscle actin mRNA in growing collateral arteries. In summary, our data showed an augmented expression level of genes contributing to different fundamental processes of arteriogenesis. (C) 2002 Elsevier Science (USA). All rights reserved.